mtDNAprofiler: A Web Application for the Nomenclature and Comparison of Human Mitochondrial DNA Sequences,

Mitochondrial DNA (mtDNA) is a valuable tool in the fields of forensic, population, and medical genetics. However, recording and comparing mtDNA control region or entire genome sequences would be difficult if researchers are not familiar with mtDNA nomenclature conventions. Therefore, mtDNAprofiler, a Web application, was designed for the analysis and comparison of mtDNA sequences in a string format or as a list of mtDNA single‐nucleotide polymorphisms (mtSNPs). mtDNAprofiler which comprises four mtDNA sequence‐analysis tools (mtDNA nomenclature, mtDNA assembly, mtSNP conversion, and mtSNP concordance‐check) supports not only the accurate analysis of mtDNA sequences via an automated nomenclature function, but also consistent management of mtSNP data via direct comparison and validity‐check functions. Since mtDNAprofiler consists of four tools that are associated with key steps of mtDNA sequence analysis, mtDNAprofiler will be helpful for researchers working with mtDNA. mtDNAprofiler is freely available at http://mtprofiler.yonsei.ac.kr .     

Concordance Between the AmpFℓSTR® MiniFiler™ and AmpFℓSTR® Identifiler® PCR Amplification Kits in the Kuwaiti Population

Abstract:  The AmpFℓSTR^® MiniFiler™ polymerase chain reaction amplification kit, developed and supplied by Applied Biosystems, complements the AmpFℓSTR^® Identifiler^® polymerase chain reaction amplification kit (Applied Biosystems, Warrington, U.K.) by improving the success rate when profiling DNA that is degraded or contains inhibitors. Before applying the MiniFiler™ kit to casework, the profiles from 200 unrelated Kuwaitis were compared to Identifiler^® profiles. Concordance was observed for 99.875% (1598 of 1600) of the compared STR loci. The two discordant profiles displayed allelic dropout: one at the D13S317 locus due to nonamplification of allele 10 in the MiniFiler™ profile, and one at the D18S51 locus due to nonamplification of allele 18 in the Identifiler^® profile.     

Freedom in the German School System

This article describes `freedom' as a constitutional category in the German School Law. If the responsibility for schooling by the German Federal Constitution is given to the ``Bundesländer' a common frame is set by the Basic Rights of the German Federal Constitution; this constitutional frame limits the rights of the ``Bundesländer' in supervising, organising, planning and managing the schools. Basic rights of a single pupil in a school are limited by the same rights of other pupils; this is done by following the ``principle of practical concordance' and thus the freedom of the single pupil always becomes a relative one; how this principle works in a concrete conflict will be shown in this article by describing single cases. Parental Rights are also guaranteed by the German Federal Constitution in direction of the state-runned schools. The rights of the parents are equal to the state rights; so a solution of a conflict has to be found between these two actors. Rights of teachers in Germany are limited by their special status as civil servants. And if the pedagogical freedom of teachers is recognised by the school acts this does not describe a real kind of freedom for the single teacher.     

Radiographic comparison of a fractured clavicle exhibiting a pseudo-arthrosis

Bone remodeling is a natural process that is potentially problematic for radiographic comparisons because it can occur after antemortem (AM) imaging, thus interfering with the comparability of AM and postmortem (PM) radiographs from the same individual. While the effects of age-related remodeling have been studied, limited attention has been given to trauma-related remodeling with respect to radiographic comparisons. This report adds to the latter topic by presenting a case of AM clavicle fracture that developed into a pseudo-arthrosis over a 12-month period prior to the individual's death. Even though remodeling was discernable along the fracture margins, adjacent skeletal features on the PM radiograph remained unaltered and constituted compelling evidence for the identification. This case illustrates the potential of using both normal and pathological anatomy concurrently to maximize the surety of findings from radiographic comparisons.     

Neue Indikatoren zur Bestimmung der arithmetischen Regierungskonkordanz

Sowohl in der Schweizer Bundespolitik als auch in den Kantonen ist die Regierungskonkordanz ein wichtiges Merkmal der Organisation der politischen Institutionen. Sie wird in etlichen Studien sowohl zur Charakterisierung der Regierungen wie auch als Erklärungsvariable verwendet. Ziel unseres Beitrages ist es, erstens die politische und die arithmetische Konkordanz konzeptionell zu unterscheiden, und zweitens eine Messung der arithmetischen Konkordanz vorzuschlagen, die die Probleme des gebräuchlichen Indikators (Sitzanteil der Regierungsparteien im Parlament) vermeidet. Der erste Indikator (“absoluter Konkordanzgrad”) berücksichtigt namentlich Konkordanzdefizite, wo Parteien weit unter oder über ihrer Parteienstärke in der Regierung vertreten sind. Der zweite Indikator (“standardisierter Konkordanzgrad”) bezieht ferner institutionelle Gegebenheiten (kleine Regierungsgrösse, hohe Parteienzahl) ein. Werden die beiden Messungen der arithmetischen Regierungskonkordanz verwendet, um beispielsweise die Nutzung der direkten Demokratie zu erklären, zeigt sich ihre Stärke im Vergleich zum bisherigen Indikator.     

Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits

The AmpFlSTR MiniFiler polymerase chain reaction amplification kit developed by Applied Biosystems enables size reduction on eight of the larger STR loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA samples. The MiniFiler Kit amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1308 samples were evaluated with both the MiniFiler and Identifiler STR kits: 449 African American, 445 Caucasian, 207 Hispanic, and 207 Asian individuals. Full concordance between Identifiler and MiniFiler Kits was observed in 99.7% (10,437 out of 10,464) STR allele calls compared. The 27 differences seen are listed in Table 1 and encompass the loci D13S317 (n = 14) and D16S539 (n = 10) as well as D18S51 (n = 1), D7S820 (n = 1), and CSF1PO (n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift.     

Party,Regional and Linguistic Proportionality Under Majoritarian Rules: Swiss Federal Council Elections

The elections to the Swiss Federal Council in December 2015 re‐established a system of party‐centred concordance, cherished in consociational theory, consisting of two representatives of the Swiss People's Party, two Radicals, two Social Democrats and one Christian Democrat. At the same time, the government has rarely been as unbalanced in terms of the representation of Switzerland's languages and regions. The article analyses the concept of concordance with regard to both aspects of governmental inclusiveness. It also highlights the crucial role of electoral rules used in governmental elections. It argues that they resemble the Alternative Vote, a majoritarian electoral system that has been criticized in consociational theory but prescribed by the rival, centripetalist approach to power sharing.     

Analysis of Forensic Autopsy in 120 Cases of Medical Disputes Among Different Levels of Institutional Settings

Despite advances in medical science, the causes of death can sometimes only be determined by pathologists after a complete autopsy. Few studies have investigated the importance of forensic autopsy in medically disputed cases among different levels of institutional settings. Our study aimed to analyze forensic autopsy in 120 cases of medical disputes among five levels of institutional settings between 2001 and 2012 in Wenzhou, China. The results showed an overall concordance rate of 55%. Of the 39% of clinically missed diagnosis, cardiovascular pathology comprises 55.32%, while respiratory pathology accounts for the remaining 44. 68%. Factors that increase the likelihood of missed diagnoses were private clinics, community settings, and county hospitals. These results support that autopsy remains an important tool in establishing causes of death in medically disputed case, which may directly determine or exclude the fault of medical care and therefore in helping in resolving these cases.