A Comparison of Various Fixatives for Casting Footwear Impressions in Sand at Crime Scenes

Footwear examination can provide an important link between the crime scene and the suspect. Casts have been taken from snow, soil, and sand substrates to assist with the examination and to accurately depict the impression. However, there has been some discrepancy on what kind of fixative, if any, should be used with impressions present in sand. This study tested four different fixatives on three sandy substrates. Eight gross characteristics were added to a boot sole, and thirty sand impressions were created in each substrate. Except for one control set that remained untreated, the impressions were treated with a fixative agent before casting. The ninety shoe casts were examined by a qualified footwear examiner and scored based on his ability to see the eight characteristics. The results indicated that pump‐action hairspray was the most successful on play and construction sand, while beach sand impressions without any fixative scored highest.     

Standardization of Histological Procedures for the Detection of Toxic Substances by Immunohistochemistry in Dipteran Larvae of Forensic Importance

Immunohistochemistry (IHC) technique is an alternative toxicological analysis to detect drugs in insects of forensic importance, but it requires thorough histological procedures. In this study, we tested different fixatives—phosphate‐buffered paraformaldehyde 4% (PP), Carnoy's fluid (CF), Kahle's solution (KS), ethanol in different concentrations, and ethanol associated to PP and CF, time of fixation and histological processes for dipteran larvae's tissue, aiming to develop a sample preparation protocol for IHC application. A suitable fixation was achieved using PP for 12 and 24 h, CF for 3 h, 70% ethanol for 19 days, and 70% ethanol/CF for 2 h/3 h. Postfixation using negative pressure, two immersions in xylene for 30 min each, and one in xylene plus paraffin for 45 min increased tissue preservation. An immunohistochemical test for cocaine detection was performed using monoclonal benzoylecgonine antibody from mouse, peroxidase‐conjugated anti‐mouse IgG and visualized by 3,3′‐diaminobenzidine method showed these histological procedures didn't compromise antigenicity.