不同机制介导怀牛膝兴奋未孕大鼠子宫平滑肌的峰电活动

在 5 0只Wistar雌性未孕大鼠子宫角浆膜面上沿子宫纵轴埋植 1对Ag -AgCl双极电极 ,腹腔注射阿托品或异搏定或消炎痛后 ,观察注射怀牛膝 0 .6mL对未孕大鼠子宫平滑肌峰电活动的影响 ,探讨其兴奋的途径。结果表明 ,阿托品阻断胆碱能M受体后 ,腹腔注射怀牛膝水煎剂 ,子宫平滑肌峰电活动表现出明显的兴奋效应 ;异搏定阻断L型Ca2 通道 (L VDCC)后 ,腹腔注射怀牛膝水煎剂 ,暴发波的最大振幅等 4项指标均无明显变化 (P >0 .0 5 ) ,而单波的正波最大振幅和峰面积则有明显增加 (P <0 .0 5 ) ,单波的负波最大振幅则无显著变化 (P >0 .0 5 ) ;腹腔注射前列腺素合成酶抑制剂消炎痛后 ,注射怀牛膝水煎剂 ,暴发波的最大振幅等 4项指标变化不明显 (P >0 .0 5 ) ,单波的正波最大振幅等 3项指标均有明显升高 (P <0 .0 5 )。结果提示 ,怀牛膝对未孕大鼠子宫平滑肌峰电活动的兴奋作用是通过胆碱能M受体以外的其他途径实现的 ,部分可能与子宫平滑肌细胞膜上的异搏定敏感的L型Ca2 通道有关 ,部分可能与刺激前列腺素合成与释放有关     

STATEWIDE SPECIAL EDUCATION SURROGATE PARENT PROGRAMS: ENSURING QUALITY ADVOCACY TO ALL FOSTER CHILDREN WITH SPECIAL EDUCATION NEEDS

The Individuals with Disabilities Education Improvement Act (IDEIA) protects foster children's rights to have a special education decision maker. For foster children who do not have a natural or adoptive parent or a responsible adult in their life to take on this role, IDEIA requires that a special education surrogate parent be appointed by appropriate procedures. Under IDEIA, these procedures are delegated to the states. Each state must ensure that local education agencies (LEAs) delineate methods for recruiting and maintaining a pool of available special education surrogate parents. Due to differing state laws and LEA procedures, there are many discrepancies in the quality and availability of special education surrogate parents. To combat these problems, this Note proposes principles for administrative regulations establishing statewide special education surrogate parent programs by examining existing statewide programs. Administered through a state's Department of Education in collaboration with child welfare agencies, statewide special education surrogate parent programs guarantee well‐qualified decision makers who will advocate for all children eligible for special education services.     

单身男性生育权探析

生育权作为人的一项基本权利,理应受到保护,但生育权的主体范围则是一直以来争论不断的问题。单身男性是否享有生育权,目前我国法律还没有明确规定,学术界也没有形成统一认识。单身男性生育权存在有其合理性与必要性,呼吁国家建立专门的法律制度对其予以规范和保护。     

Surrogacy litigation in China and beyond

Chinese law neither generally prohibits nor expressly permits surrogacy. As there has been a massive underground surrogacy market in the country, surrogacy lawsuits have occurred from time to time. Chinese courts are called to decide a number of disputed issues regarding validity of surrogacy contract, parenthood of the surrogate child, and sole care and control of the surrogate child. This article examines the judicial solutions to these disputes through a case study, and analyses whether Chinese courts have adopted appropriate approaches in applying the existing law to surrogacy lawsuits. The article further discusses the inadequacies of Chinese law in solving surrogacy disputes and regulating surrogacy, and recommends a set of suggestions for improvement so that Chinese law may better adapt to the social demand of surrogacy.     

SARS冠状病毒纤突蛋白的分子生物学研究进展

从纤突蛋白的结构分析、受体及其表位分析三方面论述了SARS冠状病毒纤突蛋白的研究进展,并与部分其他动物冠状病毒进行了比较,从分子水平上阐述了纤突蛋白的结构、受体和表位研究概况,旨在为SARS冠状病毒的诊断、疫苗设计及基础研究提供理论依据。     

中国代孕制度之立法重构——以无偿的完全代孕为对象

我国现行规章对代孕持一概禁止的态度。由于特定形式的代孕具有权利基础,符合公序良俗,能缓和家庭矛盾,符合市民社会中“人”的影像和中国传统文化,加之域外立法例对特定形式代孕的承认,这一切,使得特定形式的代孕在我国的合法化深具可行性。我国未来代孕立法的基本原则,应坚持人工生殖技术的共有立法原则与代孕技术的特有立法原则相结合的模式,未来立法应对代孕制度的各个方面进行全面、系统的规定。     

Comparative analysis of gamma-hydroxybutyrate and gamma-hydroxyvalerate using GC/MS and HPLC

This paper describes two analytical techniques used to separate and quantify gamma-hydroxybutyrate (GHB) and gamma-hydroxyvalerate (GHV). The first technique was a N,O-bis(trimethylsilyl)triflouro-acetimide-trimethylchlorosilane derivatization, followed by gas chromatography/mass spectrometry analysis using an HP-5 capillary column at a rate of 1.0 mL/min with a run time of 9.25 min. This technique was found to be sensitive (LOD 1 pg on column) and gave a low average error (5%) in a beverage study. When supplemented by a surrogate spike, the method yielded 97% analyte recovery from beverages. The second technique was high-performance liquid chromatography/UV (HPLC/UV) using a C-18 column with a (20:80% v/v) methanol:dibasic phosphoric buffer (10 mM, pH 3) at a rate of 1.00 mL/min with a run time of 7.5 min. UV detection occurred at 254 nm. This method was found to be less sensitive (LOD 0.05 microg on column) for direct analysis of aqueous samples. To remove interferences seen in the beverage study, a liquid-liquid extraction before HPLC analysis was tested. However, a decreased sensitivity (LOD 100 microg on column) and irreproducible peak profiles resulted.     

UFLC法测定新型“spike99”香料中JWH-073的含量

目的 超快速液相色谱法测定新型“spike99”香料中JWH-073的含量.方法 采用Shim-pack XR-ODSⅡ色谱柱(2.0mm×75mm,2.2μm);流动相:乙腈(含0.1％甲酸):水(含0.1％甲酸)(80∶20,v/v),流速:0.25 mL/min;检测波长280nm,柱温:35℃,进样量:2μL,外标法定量测定JWH-073的含量.考查方法的线性范围、灵敏度、精密度、回收率和稳定性,并用于检测实际案例样品.结果 JWH-073浓度在1～50μg/mL范围内线性良好(A=9 398.7C-635.75,r =0.999 9);最低检测限为100ng/mL(S/N=3);低、中、高3种浓度的加样回收率(n=3)分别为100.52％(RSD=0.36％),99.52％(RSD =0.43％),99.03％(RSD =0.17％).结论 本法测定新型“spike99”香料中JWH-073含量操作简便、灵敏、准确,重复性好,适于在相关案件检验中选用.     

IBV青岛腺胃分离株S1纤突蛋白基因的克隆与鉴定

参考Genebank发表的传染性支气管炎病毒(IBV)S1纤突蛋白基因序列,自行设计合成了1对引物,对IBV青岛腺胃分离株(SD/97/02)RNA进行RT-PCR扩增.产物经琼脂糖凝胶电泳分析,呈现1条1657 bp的条带,将其克隆入pMD18-T载体中,并进行序列测定,证实为S1基因.将此重组质粒命名为pMDQXS1.     

论代孕母的合法化基础

代孕母自出现以来因其有效的社会功能得到快速发展,同时因其衍生过多的法律伦理问题而在各国遭到不同程度的限制,有的则予以全面禁止.我国也是禁止代孕母的国家之一.禁止代孕是否符合法理精神?立足于代孕母合法化的国际潮流,从法对人的基本权利的限制、无伤害不禁止原则、正义原则及维护人的追求幸福权和家庭权几个方面深入论证了代孕母合法化的法学基础.