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1.
ABSTRACT

Heavily influenced by broad definitions of crime developed by a few pioneering critical criminologists, the main objective of this article is to provide evidence showing that the current Trump administration is a regime that commits crimes of the powerful and facilitates some types of interpersonal violence in private places. Special attention is devoted to direct and in-direct state-perpetrated violent crimes against women.  相似文献   
2.
为了确定猪生殖与呼吸综合征病毒GP5蛋白A、B抗原表位之间的相互关系和对免疫的影响,应用PCR方法扩增出A、B抗原表位基因并分别克隆于原核和真核表达载体中。原核重组质粒表达的重组蛋白经Western-blotting检测证明,该重组蛋白具有良好的抗原性。将重组蛋白和真核表达质粒免疫小鼠,前者可诱导产生针对PRRSV的特异性ELISA抗体,但是此抗体没有中和活性;后者不能诱导免疫小鼠产生PRRSV特异性抗体。结果表明,中和抗体的产生不但与A、B表位的空间构象有很大的关系,如果蛋白质分子质量过小也不能诱导机体产生有效的免疫反应。  相似文献   
3.
江苏省猪高热病病例主要继发感染病毒的多重PCR检测   总被引:11,自引:0,他引:11  
应用建立的2个多重PCR方法,对2005~2006年收集于江苏省的211份猪高热病疑似病料进行了检测。结果,PRRSV阳性率为51.2%,PCV2阳性率为44.1%,CSFV阳性率为10.4%,PPV阳性率为3.9%,PRV阳性率为2.4%。2005年病料中PCV2阳性率为21.2%,2006年为73.2%;2005年PRRSV阳性率为38.1%,2006年为67.7%。2006年猪高热病疫情比2005年严重,PCV2和PRRSV可能在其中发挥了协同致病作用。对10个PRRSV分离株ORF5基因和Nsp2基因进行的测序表明,2006年分离株Nsp2基因中有连续87个碱基的缺失。  相似文献   
4.
将猪生殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)单感染和共感染的6周龄仔猪,于感染前和感染后第3、7和10 d,采用血常规法、SWC3a单色流式细胞术以及CD3/CD4/CD8三色流式细胞术分析了感染猪外周血中白细胞、粒细胞、单核细胞、NK细胞、γδT细胞的含量。结果,感染后第3 d和第7 d,PRRSV感染组、PCV2感染组以及PRRSV+PCV2共感染组猪的白细胞、单核细胞、粒细胞、NK细胞、γδT细胞总数显著下降,并且共感染组比单感染组下降更加严重。结果表明,PRRSV+PCV2共感染对仔猪外周血天然免疫细胞的影响具有协同效应,意味着在感染早期可导致更加严重的先天免疫抑制。  相似文献   
5.
Denmark has become a destination for single women, lesbians, and heterosexual couples wanting donated sperm. At the moment, women from Sweden, Norway, Germany, Italy, and the UK travel to Denmark. Simultaneously, waiting lists for donated eggs and age restrictions are prime motivations for infertile Danish women and heterosexual couples to leave Denmark and travel to Spain, the Czech Republic, the Ukraine, and Greece for egg donation. Informed by Donna Haraway’s notion of “the apparatus of bodily production”, Marcia Inhorn’s development of “reproductive flows”, and the use of Adele Clarke’s “situational analysis”, this paper explores the question: How do global reproductive pathways in and out of Denmark emerge when fertility travellers narrate, negotiate, and cross national borders to go through fertility treatment? Methodologically, we use a multi-sited and multi-modal approach centring on interviews with fertility travellers moving to and from Denmark in combination with ethnographic observations carried out in Danish and Spanish fertility clinics and an analysis of legal regulations. The paper concludes by discussing how the concept of reproductive pathways helps to theorize transnational movements of bodies and contributes to feminist scholarship on transnational reproductive travel.  相似文献   
6.
柴玉洁 《学理论》2009,(9):129-131
莫言的作品在历史背景下述说着中华民族的过往,它能深入到我们选择性遗忘的历史图景让我们在其作品的死亡意识和生殖崇拜下去解读莫言及其作品的生命意识,他以鲜明的色彩,大胆的叙述去体悟大地民间的生命强力,莫言代大地立言,去为失去话语权的人们唱响生命的悲歌。  相似文献   
7.
This article builds on the recent debates on human rights and development to discuss the case of reproductive rights and midwifery activism as part of the broader mobilisation for the humanisation of birth and against obstetric violence in Latin America and Mexico. Drawing on extensive fieldwork, the analysis shows how human rights continue to form a significant contentious and constructed terrain among women in the global South. The mobilisation for the humanisation of birth and against obstetric violence indicates how the clinical developmental view of reproductive rights is challenged by these activists as not necessarily safeguarding the rights of women during birth. In Mexico this campaign is essentially linked to the struggle to bring back and strengthen midwifery as a way of ensuring improved human rights in birth. The article concludes, however, that this campaign might be challenged by Indigenous rights in the near future.  相似文献   
8.
将猪生殖与呼吸综合征病毒(PRRSV)CH-1a株GP5蛋白基因信号肽部分删除后克隆于pGEX-6p-1载体上,并在大肠埃希氏菌中进行表达。经SDS-PAGE电泳分析发现,融合表达的rtGP5蛋白约42 ku,将融合蛋白rtGP5用PRRSV阳性血清进行Western-blotting分析,证实所表达的融合蛋白能被其特异性识别。收获融合表达产物rtGP5,按50μg/只的剂量与等量弗氏佐剂乳化,经腹腔免疫BALB/c小鼠3次后,取脾细胞与SP2/0骨髓瘤细胞进行融合,分别以融合蛋白rtGP5和GST蛋白为抗原,通过间接ELISA方法对融合细胞的上清液进行检测,筛选阳性克隆,结果得到了15株能稳定分泌抗rtGP5蛋白抗体的阳性细胞克隆株。经间接免疫荧光检测发现,获得的15株单克隆抗体都能与PRRSV感染细胞产生特异性荧光。15株单克隆抗体亚型鉴定结果显示均为IgG1型,且轻链均为κ链。  相似文献   
9.
10.
This article examines how the recently introduced law on assisted reproduction in Italy, which gives symbolic legal recognition to the embryo, came about, and how a referendum, which would have repealed large sections of it, failed. The occupation of the legal space by the embryo is the outcome of a crusade by a well-organised alliance of theo-conservatives. These groups see in reproductive medicine an uncontrolled interference with their notion of the natural order of things. Such a worldview requires a total ban on stem cell research, limitation of access to reproductive technologies and repressive laws to govern the area. This conservative dream scenario has come closer to being realised by the introduction of a law doing all of these things in the name of the protection of “Life”. In the case of this law, the “life” to be protected is the embryo. In the name of “Life”, scientific advances and individual liberty have been curbed. The politics of embryo citizenship is a politics which values the yet to come over the here and now, purgation over pleasure, and the transcendent over the material.  相似文献   
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