LASTING PEACE A decades-old international relations tenet remains crucial to global security

正Although China is demonstrating its peaceful approach to international affairs,it has always been an easy target for critics in the Western media.Any unharmonious interaction between China and its neighbors is likely to be exaggerated by the outside world as China’s wrongdoing.Observers call it undergoing the"growing pains"of a giant.Regardless,China is trying to     

宪政视角下的大部制改革

大部制改革不仅标志着中国宪政建设的新阶段,而且标志着宪政理论发展的新高度。宪政视角下的大部制改革,既是关于国家机关实行精简原则并反对官僚主义的宪法规定在行政体制内的自我实现,也是在行政权系统之外的综合性政治体制改革,这恰恰是行政管理体制改革走出"精简—膨胀—再精简—再膨胀"这一怪圈的根本出路。     

Multiple Giant Coronary Artery Aneurysms: A Rare Cause of Sudden Cardiac Death

Multiple giant aneurysms involving the coronary arteries are uncommon and rarely reported. In the presented case, a 63‐year‐old man with poorly controlled hypertension died suddenly. Gross autopsy examination showed multiple giant thrombus‐filled coronary artery aneurysms, atherosclerotic coronary artery disease, and cardiomegaly. Histological sections of the coronary aneurysms showed atherosclerotic changes with both organized and fresh thrombus. Giant coronary aneurysm is defined as a segmental enlargement of a coronary artery with a diameter exceeding 20 mm or more. The main etiology of this nebulous entity is attributed to atherosclerosis and inflammatory or inherited connective tissue disorders with the remainder being congenital, infectious, or idiopathic. Before its cataclysmic presentation, when ruptured or thrombosed, giant coronary aneurysm usually has a silent clinical course. Sudden death owing to giant multiple coronary aneurysms is rare and mandates careful classification of the aneurysms and prudent search for autoimmune‐mediated or genetically based factors for subsequent ancillary autopsy studies.     

大熊猫轮状病毒CH-1株外衣壳蛋白VP4基因的克隆与生物信息学分析

利用MA-104细胞培养增殖大熊猫轮状病毒CH-1株,提取总RNA,运用RT-PCR扩增外衣壳蛋白VP4基因,将其与pMD19-T simple vector连接并转化DH5α,经PCR扩增和测序分析进行鉴定,用生物信息学软件预测其功能,并构建系统进化树。结果显示,成功获得大熊猫轮状病毒VP4蛋白基因,长2 362bp,包含一个2 331bp的开放阅读框,编码776个氨基酸,测序结果在GenBank中的登录号为HQ641296。生物信息学分析表明,VP4蛋白基因编码产物分子质量为86 768.8u,理论等电点为5.56,半衰期为30h(体外哺乳类网状细胞),不稳定系数为28.64,脂肪指数为82.53,总平均亲水性为-0.265,最大疏水性为2.244,最小疏水性为-2.911;无信号肽序列;跨膜结构分析显示VP4蛋白无跨膜区,位于病毒膜外区;在VP4序列中的抗原决定簇主要位于VP4的N-末端和C-末端。预测其可能包含8个N-糖基化作用位点,15个蛋白激酶C磷酸化作用位点、15个酪蛋白激酶Ⅱ磷酸化作用位点、12个N-豆蔻酰化位点。系统进化分析显示,大熊猫轮状病毒的VP4基因与猪轮状病毒的VP4基因的进化距离最...     

大熊猫源停乳链球菌类马亚种的分离与鉴定

为研究引起大熊猫脚掌化脓感染的原因,有效防治该病,对大熊猫感染部位的浓汁进行了细菌学检查及分离,对分离菌进行形态特征、理化特性鉴定、兰氏分群以及16S rDNA序列的分子鉴定,并构建系统发育树;对分离菌进行健康小鼠的人工感染试验。结果分离到5株细菌,根据5株分离菌的形态特征、理化特性及兰氏分群结果,结合16S rDNA序列测定与系统发育分析结果,判定为链球菌属的停乳链球菌类马亚种。该分离菌对供试健康小鼠有较强的致病作用。证实分离菌即为引起大熊猫脚掌化脓感染的病原菌。     

大熊猫IgG Fc HRP酶标二抗的制备

为获得辣根过氧化物酶(HRP)标记的抗大熊猫IgG抗体,本研究利用重组大肠杆菌表达技术对大熊猫IgG Fc片段进行了表达,获得重组蛋白后进行了小鼠免疫,抗体特异性检测,并进行了多克隆抗体的HRP标记.结果显示,在37℃、IPTG浓度为0.5mmol/L、诱导5h的条件下,可获得分子质量约为88 ku的目的蛋白;将重组蛋...     

Doing Justice to Anti-Trust Actions

正Since July,Chinese regulators have launched anti-trust investigations into several well-known foreign companies operating in China,including telecommunications company Qualcomm,computing giant Microsoft Corp.and Audi dealers under the auspices of FAWvolkswagen Automobile Co.Ltd.     

社会主义文化强国与青年学者历史责任

加强理论创新,推进哲学社会科学发展繁荣是建设社会主义文化强国赋予青年学者的历史使命,必须重视和解决好困扰青年学者履行历史责任的问题,重点是要增强青年学者责任感与使命感,提高治学水平和学术修养,实现个人价值与社会价值的统一;探索和创新青年学者培养模式;引导青年学者关注现实问题研究,用科学理论指导解决好经济社会发展中的重点、难点问题;完善学术研究机制,引导青年学者学术研究的理性回归。     

不同季节亚成体大熊猫肠道菌群ERIC-PCR指纹图谱分析

为探讨不同季节亚成体大熊猫肠道菌群的相似性和稳定性。对采自雅安碧峰峡大熊猫基地3只亚成体大熊猫的粪便细菌总DNA进行了ERIC-PCR指纹图谱分析,并对ERIC-PCR指纹图谱中主带DNA片段进行回收、克隆测序及序列比对分析。结果显示,同一季节不同亚成体大熊猫肠道菌群结构的相似性较高,而不同季节同一亚成体大熊猫肠道菌群结构的相似性较低;亚成体大熊猫肠道菌群的多样性可能受季节影响;大肠杆菌和柠檬酸杆菌为亚成体大熊猫肠道的优势细菌。表明,亚成体大熊猫肠道菌群的多样性在一定程度上与季节有关。     

Sudden cardiac death due to giant cell inflammatory processes

Granulomatous inflammation of the myocardium may occur in a number of systemic disease processes including those with infectious etiologies such as fungal, mycobacterial and parasitic infections, as well as hypersensitivity reactions, and rarely autoimmune disorders. In many of these disorders, giant cells are components of the inflammatory infiltrate. Systemic granulomatous processes of unknown pathogenesis, most notably sarcoidosis, may also be associated with involvement of the myocardium. Occasionally, these disorders are associated with sudden death due to pathologic involvement of the heart. In contrast, giant cell myocarditis, also known as idiopathic myocarditis, a rare, frequently fulminant and fatal disorder of unknown etiology, is isolated to the heart and lacks systemic involvement. This disorder is most commonly diagnosed at autopsy. We present two cases in which sudden death resulted from a giant cell inflammatory process affecting the myocardium. Both individuals lacked antemortem diagnoses and collapsed at their respective places of employment. These cases compare and contrast the clinical and pathologic issues involved in the differential diagnoses of the subgroup of sudden cardiac deaths resulting from giant cell inflammatory processes that affect the myocardium, as well as the value of histologic examination and immunohistochemical studies.