鸡传染性支气管炎病毒陕西分离株M蛋白基因的变异及其B细胞表位稳定性

以陕西8株鸡传染性支气管炎病毒(AIBV)分离株M蛋白的基因序列为基础,应用DNAStar软件中的MegAlign模块对其进行了同源性分析;用Kyte-Doolittle方法、Jameson-Wolf方法、Karplus-Schulz方法、Plot-Emini方法和Garnier-Robson方法综合预测了其中4株代表毒株的M蛋白B细胞抗原表位和二级结构。结果,8株AIBV的M蛋白主要在10~40 aa和90~175 aa处存在无规律的点突变,与参考毒株H52、M41、SAIB20、LX和Gray的M蛋白的核苷酸序列和预测的氨基酸序列的同源性分别介于87.4%~99.5%和96.3%~99.5%;分离株间的核苷酸序列和预测的氨基酸序列同源性分别介于91.8%~99.8%和95.4%~100%。4株代表株的M蛋白B细胞优势抗原表位都在159~164 aa和181~193 aa肽段区(这2个区域都包含了无规则卷曲和β-转角结构)或其附近。结果表明,AIBV分离株的M蛋白基因相对保守,只存在无规律的点突变,该变异对AIBV M蛋白B细胞表位的稳定性无影响。     

知识经济的法律调整着力点初探

知识经济的运行 ,是通过知识的产权化、商品化和资本化 ,以实现知识的要素化和产业化的过程 ,与此伴随的还有知识的异化过程。法律应当以知识的产权化、商品化、资本化、要素化、产业化和异化为着力点 ,对知识经济进行调整。     

重型犯常见的心理问题及应对策略

重型犯是指犯罪性质恶劣、情节严重以及社会危害性很大的罪犯。用症状自评量表(SCL—90)对重型犯进行了测查,发现入监初期与入监中期服刑人员相比,入监中期服刑人员在躯体化、忧郁、偏执三项因子分方面明显高于入监初期犯人,表明服刑时间较长的罪犯存在更多的心理问题。因此,综合运用各种改造手段对罪犯进行心理和行为矫正,广泛开展心理健康教育,就可以极大地提高我国特殊预防的水平。     

城乡居民收入差异的区域性特点分析

通过分析城乡居民抽样调查资料,建立城乡居民收入差距的回归分析模型,对城乡居民收入差异的区域性特点进行了比较分析。从而为缩小城乡居民收入差距提供了理论参考。     

External Pressures and Internal Dynamics in the Institutionalization of Performance-Based Budgeting: An Endless Process?

The well-known practice of performance-based budgeting (PBB) is a relevant component of the New Public Management (NPM) reform agenda and has become widespread, with varying approaches and results across countries. However, its variation within specific countries has remained largely unexplored. This study analyzes three organizations operating within the same context—three ministries in Italy—to contribute to a new understanding of PBB variation by illustrating why the same PBB practice can or cannot be implemented and internalized similarly across these organizations and thus become (or not) fully institutionalized. The study adopts and enriches the institutional approach by extending beyond isomorphic convergence toward PBB and explaining practice variation, linking the interactions between external pressures and internal dynamics at the organizational level to PBB institutionalization. The empirical analysis shows how a lack of alignment between external pressures and internal dynamics contributes to an unfinished and apparently endless process of institutionalization.     

西方女权主义思想在中国的变异

西方女权主义思想在 2 0世纪的中国遭遇到尴尬 ,尤其是对“女权主义”这一外来词语形成的种种曲解或误解 ,造成了众多女性学者对于“女权主义者”立场的不认同等 ,而隐于这种现象背后的原因在于异质的中西文化。女权主义与中国特定的文化相遇和碰撞 ,产生了某种程度的变异 ,由原来激进的、女性自主的行动演变为温和的、有男权参与的、凭借社会政治手段实现的解放妇女的运动。“女权主义”与“女性主义”、“妇女解放”与“解放妇女”的差异不应仅仅被视为词语的偏正关系的变化 ,而更有着深刻的内在民族文化心理上的原因。     

浅析物权的消灭

物权的消灭在《物权法》体系中有着不可忽视的重要地位.我国《物权法》虽然规定了物权的变动,但是对物权的消灭却未有完善、全面的规定,致使国家大量资源闲置,无法得到有效利用.通过对物权的消灭进行研究,以期探究物权的消灭对中国物权法体系结构的重要意义.     

The Variation and Mutual Infiltration of Literary Field in the New Century

After entering into the new century, Chinese contemporary literature is subjected to the double pressure of the electronic media and the market economy. the independent “Elite literature” field which is dominated by the literature publishing is experiencing the variation under the influence of many factors such as politics, economy, science and technology, thought and mentality. the variation includes four secondary literature field, they are elite literature, youth literature, network literature and popular literature respectively. The field of Chinese literature is composed by the four secondary literature field since the new century, they coexist and compete mutually.This competitive situation is unavoidable for the time being, that is to say, the mutual relationship among elite literature, youth literature, network literature and popular literary is both competitive and cooperative, the image of the competition and connection with each other is the current situation of contemporary Chinese literature.     

复写纸复写笔迹变化的原因探析

与正常书写条件下形成的笔迹相比，复写纸复写笔迹的特征变化性更大，鉴定难度更高。本文阐述了从书写承受物、书写工具、书写时间、书写人心理等方面进行深入研究，探析复写纸复写笔迹变化的几点主要原因，为全面掌握复写纸复写笔迹变化的规律和准确鉴定该类笔迹打下了坚实的理论基础。     

重新定义高科技时代下的搜查

高科技搜查手段的出现，改变了传统搜查的理论依据。高空搜查、高空照相、通讯记录搜查、电波追踪、热像仪、望远镜等高科技搜查手段的使用，使判断搜查的标准发生了变化。