香港商业罪案调查的主要特点及其借鉴意义

香港作为国际性的贸易和金融中心,其商业犯罪案件具有典型性。香港警方对付商业犯罪,也具有特点。主要是有完善的易于操作的法例,能动的侦查机制,网络化的联动查案方式以及高度专业化的人力支援等。这些方面对于中国大陆都有借鉴意义。     

Does online voting change the outcome? Evidence from a multi-mode public policy referendum

Do online and offline voters differ in terms of policy preferences? The growth of Internet voting in recent years has opened up new channels of participation. Whether or not political outcomes change as a consequence of new modes of voting is an open question. Here we analyze all the votes cast both offline (n = 5.7 million) and online (n = 1.3 million) and compare the actual vote choices in a public policy referendum, the world's largest participatory budgeting process, in Rio Grande do Sul in June 2014. In addition to examining aggregate outcomes, we also conducted two surveys to better understand the demographic profiles of who chooses to vote online and offline. We find that policy preferences of online and offline voters are no different, even though our data suggest important demographic differences between offline and online voters.     

H5N1亚型禽流感病毒NS1基因的克隆及表达

利用RT-PCR扩增了2株H5N1亚型禽流感病毒NS1基因,并将其克隆到pMD 18-T载体上,进行序列分析。结果显示,这2株禽流感病毒NS1基因核苷酸序列的同源性为70.2%,分别属于NS等位基因群A和等位基因群B。再将克隆的NS1基因插入到pET-28a质粒中构建原核表达载体,将其转化到DH5α大肠埃希氏菌感受态细胞中,经双酶切鉴定及序列分析,表明获得了重组质粒pET-52NS1和pET-174NS1。经SDS-PAGE分析,重组质粒转化BL21(DE3)(pLysS)感受态细胞后,经IPTG诱导,获得了分子质量约为30 ku的NS1融合蛋白。用AIV多克隆血清做Western-blotting分析,发现来自2个等位基因群的NS1蛋白都具有较好的抗原活性。     

以“三创一办”为平台 努力践行“贵阳精神”

"贵阳精神"作为贵阳的城市灵魂和发展动力,必须在推进城市发展的实践中才能充分体现其核心价值。目前正在广泛开展的"三创一办"为践行"贵阳精神"提供了一个难得的机遇和平台。     

专业安全应用为本

本文讨论了在信息化高速发展的现代,我们应当以一种怎样的手段来应对各种层出不穷的威胁.进而阐述H3C的iSPN理念.     

Vergleichswiderruf durch Telefax beim falschen Gericht

Erfolgt die Bekanntgabe eines Vergleichswiderrufs wie vereinbart durch Telefax, aber an die Telefaxnummer eines falschen Gerichts, so wahrt dies die Frist für den Widerruf nicht, wenn das Telefax tats?chlich erst versp?tet bei der Einlaufstelle des zust?ndigen Gerichts einlangt; m?gen sich auch beide Gerichte im gleichen Geb?ude befinden.     

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.     

H7N2禽流感病毒分离株血凝素蛋白全基因的序列分析

为进一步分析H7N2禽流感病毒(AIV)分离株血凝素(HA)基因的特性,参照已发表序列设计了1对引物,采用RT-PCR获得了1条约1.7 kb的DNA片段,测序后进行了同源性比较、HA基因系统发育进化树分析和氨基酸编码分析。结果表明,所测的2个分离株的HA基因全长1 664 bp,编码除信号肽以外的HA蛋白的全部544个氨基酸,其中包括HA1的323个氨基酸,HA2的221个氨基酸。2个分离株HA基因核苷酸序列的同源性为99.4%;与GenBank中AIV标准株A/Afri.Star./Eng-Q/983/79(H7N1)的同源性最高,分别为99.4%和99.0%;与美国A/Chicken/NewYork/13142-5/94(H7N2)株同源性很低(仅65.0%),而与以色列、意大利H7N2AIV的同源性较高(为96%~97%);2个分离株在HA基因进化树中均处于H7亚型AIV的欧亚群系分支内。推导氨基酸的序列分析表明,其HA蛋白裂解位点的氨基酸序列为-GR-GLF-,仅包含1个碱性氨基酸(R-)残基,符合低致病力AIV的基因特征。     

试论托尼的平等观

平等思想历来是中外思想家所关注和深入研究的大问题,英国近代思想家托尼是研究平等思想的著名学者,他从天赋、平等对待和平等推进等角度极赋见地地论述和阐发了平等思想,他的所有政治构想、经济研究和对社会、历史的探索都是围绕平等展开的。可以说平等是托尼全部政治思想的基础和核心。     

H9N2亚型禽流感病毒聚合酶基因的克隆及序列分析

采用RT-PCR方法对1株H9N2亚型禽流感病毒Ck/GS/2/99株RNA聚合酶基因PB2、PB1和PA分别进行了扩增,将其克隆到pGEM-T easy载体后进行序列测定与分析。结果表明,该株病毒的PB2、PB1和PA基因开放阅读框(ORF)分别由2280、2274和2 151个碱基组成,分别编码759、757和716个氨基酸。经同源性与系统发生树分析,该毒株RNA聚合酶基因PB2、PB1和PA与Ck/NX/4/99株的核苷酸和氨基酸同源性均在98%以上,另外这3个基因与Dk/HK/Y280/97、Sw/HK/9/98和Qu/HK/NT/28/99株的关系密切。