汉族人群五个STR基因座的多态性调查

应用PCR及PAG电泳技术研究了PLA2A、vWA、CYP19、TH和LPL五个基因座的多态性，调查武汉地区汉族无关个体，获得汉族人群的频率分布。五个基因座基因型频率分布与Hardy-Weinberg平衡吻合良好、分别计算基因座的杂合度（H）、个人识别能力（Dp）、非父排除率（PE）和多态性信息总量（PIC）。为法医学应用提供了基础数据。     

用引物Y_3,Y_4和DNA扩增技术作血液(痕)和毛根性别鉴定的研究

作者用引物Y_3、Y_4和DNA聚合酶链式反应(PCR)作微量人类血液(痕)和毛根的性别鉴定。扩增的靶序列位于Y染色体DNA特异3.4kb重复序列中,扩增产物为460bp。检材用量为:新鲜血液0.5μl、血痕纱纤维1mm、毛根单个。20例保存4个月的血痕与2例保存6年半的血痕性别判定结果均正确,无性别记载的保存9～11年的3例血痕显现了清晰的460bpY特异DNA扩增带。15例保存20天的自然脱落毛根性别判定结果均正确。本法省略了检材处理中的酚-氯仿抽提DNA等纯化步骤,既简化了实验操作,又减少了检验过程中外源DNA的污染机会和样品DNA的损耗,使这一性别鉴定方法更符合法医学实践的需要。     

中国5个群体D20S85基因座的遗传多态性

目的 了解中国5个群体D20S85基因座的群体遗传学数据,比较它们之间的遗传学差异,探讨其在法医学应用中的意义。方法 分别收集5个群体622名无关个体的血样,Chelex-100快速抽提法或饱和酚/氯仿法抽提DNA;扩增后经PAGE垂直板电泳、银染,进行D20S85基因座分型。结果 在5个群体622名无关个体中,共检出9个等位基因,并首次在广东汉族和广西壮族群体中检出等位基因14;每个群体基因频率大于0.05的均为6个,D20S85*6为最常见等位基因。5个群体共观察到35种基因型,群体内基因型频率分布均符合Hardy-Weinberg氏平衡,各群体间基因型构成比无显著性差异。观察140次减数分裂未发现突变。各群体的期望杂合度为0.7720～0.7912;非父排除率,在三联体为0.7538～0.7594,二联体为0.3988～0.4297;个人识别率为0.9175～0,9272;多态信息含量为0.7442～0.7656。应用于亲子鉴定和个人识别案例,效果满意。结论 D20S85基因座是法医学应用价值较高的遗传标记系统。     

虚拟价值链的理论与应用

本文详述了虚拟价值链的思想 ,并尝试构建出虚拟价值链的一般理论模型 ,从而解决了企业在信息时代的竞争中缺少战略指导的缺陷。通过论述虚拟价值链和传统价值链的关系 ,本文指出了虚拟价值链模型的适用范围 ,并提出了企业应用这一模型进行竞争的基本原则和方法。     

Are Power Asymmetries a Reason for the State to Intervene?

Power asymmetries between farmers and slaughterhouses have led the Swiss government to install an independent agency that evaluates the quality of pig and cattle carcasses. This case is used to explore public interventions to mitigate power asymmetries. The independent classification is described and its economic rationale explored. The positions of the most important stakeholders in the system are depicted and the counter-arguments analyzed by objective hermeneutics. It is concluded that public interventions against power asymmetries are no panacea but can be useful to create a just and effective system.     

证据保管链制度研究

证据保管链制度要求建立自侦查阶段收集证据至审判阶段将证据提交法庭的完整记录体系;除少数例外情形,所有接触证据的人员都必须出庭作证。这一制度对规范侦查、起诉人员收集、运输、保管证据等行为,协助法官和辩护方审查判断证据的真伪,都具有极为重要的意义。我国刑事诉讼法只要求对证据的收集进行记录,而不要求对证据的运输、保管、鉴定等也进行记录,更不要求接触证据的人员出庭作证;这对保障实物证据的证明力极为不利。我国在未来立法时有必要借鉴域外经验,建立系统的证据保管链制度。     

941方对衰老大鼠肝线粒体酶活性的影响

目的：通过检测衰老大鼠肝组织细胞线粒体呼吸链氧化功能。探讨大鼠衰老时肝组织细胞线粒体呼吸甸氧化功能的变化规律及941方延缓衰老的作用机制。方法：分别从4月龄（青年组）、24月龄（老龄组）和941方组大鼠的肝组织分离出线粒体,并用双缩脲法测定单位组织的线粒体蛋白,用Clark氧电极极谱法分别测定线粒体呼吸链3个氧化酶活性：NADH氧化酶（NADHOX）、琥珀酸氧化酶（SUCOX）和细胞色素氧化酶（CYTOX）。用差光谱法（联二亚硫酸钠还原减正铁氢化钾氧化的消化值）获得细胞色素a、b、c和c1含量（Cyta、Cytb、Cytc和Cytc1)。结果：与青年组大鼠比较,老年组大鼠的肝组织细胞线粒体含理有一定下和,百3个氧化酶活性却显增加,同时细胞色素含量尤其是Cytb和Cytc显升高,941方组大鼠肝组织细胞线粒体上述指标皆显高于青年组和老年组。结论：衰老大鼠肝组织线粒体酶活性与青年组有显不同,表明衰老过程中肝细胞通过某种反馈机制使残存完好的线粒体氧功能代偿性增强;９４１方使这种作用进一步增强。     

浅谈电子商务与房地产供应链管理

电子商务的兴起对供应链管理的现代化提供了有效的手段,并将对各个行业的运作模式产生巨大的冲击。利用电子商务改造房地产业务供应链可以有效地整合供应链上的多种资源,提高产业效率,并且便利了政府对房地产业的管理,因此已经成为房地产业务供应链管理的新趋势。     

Simplified Direct PCR Method for Reference Buccal Samples Using a Non-FTA Card by Omitting the Pretreatment Step

When using non-FTA cards in commercial multiplex STR kits for direct PCR, pretreatment steps with specific buffers are recommended. Here, we designed a rapid direct PCR method utilizing a non-FTA card, Oral Cell Sampling Kit, by omitting the pretreatment step involving Prep-n-Go™ Buffer, and it showed compatibility with the GlobalFiler™ Express PCR Amplification Kit, GlobalFiler™ PCR Amplification Kit, and PowerPlex^® Fusion system. To optimize the PCR conditions, we tested the method with different final PCR volumes and cycles. Finally, we conducted a performance test using 50 Korean buccal samples and confirmed the high performance of the method, detecting more than 90% of the samples with full profiles when using GlobalFiler™ PCR Amplification Kit and PowerPlex^® Fusion system at 29 cycles in a 10 μL final PCR volume. Thus, we report a simple direct PCR set-up to analyze reference samples collected using a non-FTA card manufactured in Korea.     

Governing sustainable palm oil supply: Disconnects,complementarities, and antagonisms between state regulations and private standards

The global palm oil value chain has grown in complexity; stakeholder relationships and linkages are increasingly shaped by new public and private standards that aim to ameliorate social and environmental costs while harnessing economic gains. Regulatory initiatives in the emerging policy regime complex struggle to resolve sector‐wide structural performance issues: pervasive land conflicts, yield differences between companies and smallholders, and carbon emissions arising from deforestation and peatland conversion. Identifying opportunities for more effective governance of the palm oil value chain and supply landscapes, this paper explores disconnects, complementarities, and antagonisms between public regulations and private standards, looking at the global, national, and subnational policy domains shaping chain actors’ conduct. Greater complementarities have emerged among transnational instruments, but state regulation disconnects persist and antagonisms prevail between national state regulations and transnational private standards. Emerging experimental approaches, particularly at subnational level, aim to improve coordination to both enhance complementarities and resolve disconnects.