油樟叶挥发油对三种真菌的抗菌效果

为探讨油樟叶挥发油对须毛癣菌、犬小孢子菌和石膏小孢子菌的抑制活性及其作用机理,采用RPMI 1640液体培养基倍比稀释和沙氏琼脂平板稀释培养计数法分别检测油樟叶挥发油对这3种真菌的最小抑菌浓度(MIC)和最小杀菌浓度(MFC);并绘制体外杀菌曲线,观察MIC浓度下油樟叶挥发油对3种真菌的动态杀菌过程;透射电镜下观察油樟叶挥发油在MIC浓度下对3种真菌超微结构的影响。结果显示,油樟叶挥发油对3种真菌的MIC和MFC值均为3.125μL/mL;杀菌曲线表明,作用的前12h内油樟叶挥发油对3种真菌的杀菌作用最强,随后杀菌作用降低;电镜下对照组的3种真菌形态正常,在MIC浓度下,3种真菌原有的细胞器模糊不清、溶解或消失。试验证实,油樟叶挥发油对3种真菌具有较强的杀菌作用,其杀菌机理为破坏真菌的细胞器。     

Childcare Policies and the Politics of Choice

Governments have intervened in two basic ways on childcare: via the provision of leaves to care (usually taken by mothers) and the provision or (often only partial) financing of childcare services. These policy options reflect the fundamental debate on how young children should be cared for. Labour has developed both policy areas since 1997, but there is a question mark over how far mothers and fathers have a ‘real choice’ to work and/or to care. Parents' choice in this policy area is a sensitive political issue and, this article suggests, requires a careful balancing of policy instruments. Policy goals may conflict with preferences and there is also the difficult issue that parents' choices may prejudice their future welfare in a work/welfare system that is tending to assume that there will be increasing capacity for self‐provisioning.     

Suicidal yew ingestion

A 41-year-old man was found dead, lying in prone position on the ground near a parking area, where the car of the person was parked. Remarkable were the spasm-like hands of the decedent, cause of death was unknown. Primary an epileptical attack was assumed. A medicolegal autopsy was performed followed by histological and forensic toxicological analysis. Furthermore a plant anatomical investigation was applied. Autopsy revealed fragments of greenish needle-like leaves in the stomach and the intestines, but not in the colon. Plant anatomical investigation indicated the presence of yew. Histological findings were in concordance with the literature, in addition the investigation of bone marrow was performed for the first time. Forensic toxicological analysis revealed the presence of 3,5-dimethoxyphenol, the marker for intoxication with taxus baccata.This case reports the importance of medicolegal examinations in order to avoid misdiagnosis. Here the suspection of an epileptical attack as a possible cause of death was excluded by an autopsy, in addition an intoxication by yew leaves was proved.     

油樟叶乙醇提取物抗炎活性的研究

为探讨油樟叶乙醇提取物的抗炎活性,建立了用二甲苯诱导小鼠耳肿胀、醋酸诱导小鼠毛细血管通透性增加和角叉菜胶诱导大鼠足肿胀的模型,并测定了角叉菜胶所致大鼠足炎性渗出液中前列腺素E2、组胺和5-羟色胺的含量。结果表明,油樟叶乙醇提取物能显著抑制二甲苯所致的小鼠耳肿胀、醋酸诱导的小鼠毛细血管通透性增加和角叉菜胶所致的大鼠足肿胀,且呈剂量依赖性,并能显著降低角叉菜胶所致大鼠足炎性渗出液中前列腺素E2、组胺和5-羟色胺的含量。证实油樟叶乙醇提取物具有较好的抗炎活性。     

白鹃梅嫩叶及花序中重金属和农药残留检测

目的 对白鹃梅嫩叶及花序的重金属和农药残留量进行测定,为白鹃梅嫩叶及花序的进一步开发利用提供参考.方法 运用原子吸收分光光度法、原子荧光光度计法和气相色谱法测定白鹃梅嫩叶及花序中重金属和农药残留的含量.结果测得直接晒干的白鹃梅嫩叶及花序中砷、铅、铜的含量分别为0.147 1、3.399 7、0.187 4 mg/kg,...     

对董事离任义务立法现状的分析及建议

我国现行《公司法》只对董事的在任义务作了规定,而未涉及到董事的离任义务。随着社会经济活动的多元化和复杂化,越来越要求在《公司法》中对董事的离任义务作出规定。应在《公司法》中明确规定董事离任后的竞业禁止义务、禁止篡夺公司机会义务、保守秘密义务、不得与公司从事特定财产交易义务、不得策反公司重要职员义务、不得随意转让其所持股份之义务。     

菊茎叶总黄酮高效液相色谱指纹图谱与抗氧化活性的谱效关系研究

目的 研究菊茎叶总黄酮指纹图谱与其抗氧化活性的相关性,探究菊茎叶总黄酮抗氧化活性的物质基础。方法 采用高效液相色谱法建立11批菊茎叶总黄酮样品的指纹图谱;分别采用1,1-二苯基-2-三硝基苯肼自由基清除法、2,2′-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基清除法及血浆铁离子还原法测定其抗氧化活性,结合灰色关联度法、偏最小二乘回归法分析谱效关系。结果 建立17个共有峰的菊茎叶总黄酮的高效液相色谱指纹图谱,指认其中11个共有峰,相似度大于0.886。灰色关联分析显示其中11个色谱峰关联度均大于0.9,偏最小二乘回归分析结果显示6个色谱峰的变量重要性投影值>1,表明菊茎叶总黄酮与抗氧化活性有重要相关性。结论 共有峰6（山柰酚-3-O-芸香糖苷）、峰11、峰12（香叶木素-7-O-β-D-葡萄糖苷）、峰13（木犀草素）、峰15与抗氧化活性密切相关,初步推测为菊茎叶总黄酮抗氧化活性的物质基础。     

安徽银杏资源调查

目的：调查安徽银杏的分布与资源。方法：对全省银杏分布及现代栽培基地进行实地考察。结果：发现安徽是银杏自然分布中心区之一，也是栽培较为集中的地区，资源丰富。结论：产区应进一步开展利用银杏资源，并加强银杏栽培管理。     

油樟叶挥发油水乳剂对家兔自然感染皮肤真菌病的疗效观察

从患皮肤真菌病家兔的皮屑、被毛中分离病原真菌,并进行病原鉴定;分别用5、10、30mL/L油樟叶挥发油水乳剂通过皮肤涂擦法进行治疗试验,以观察油樟叶挥发油水乳剂对家兔皮肤真菌病的疗效。结果显示,分离得到的2种病原真菌经培养特性、菌落性状、菌丝形态和孢子性状特征等鉴定,为须毛癣菌和犬小孢子菌;3种剂量组的油樟叶挥发油水乳剂对家兔皮肤真菌病均有较好的疗效,其中以10mL/L油樟叶挥发油水乳剂对于家兔自然感染皮肤真菌病的疗效最好。试验表明油樟叶挥发油水乳剂对兔自然感染的皮肤真菌病具有一定的疗效。     

银杏叶提取物对豚鼠肠系膜下神经节细胞生物电的影响

目的：通过观察银杏叶提取物(EGb)对交感神经节细胞生物电的影响，为进一步探讨EGb调节肠系膜下神经节(IMG)支配的包括远端结肠在内的内脏功能活动的作用机制提供依据。方法：采用细胞内生物电记录技术记录体外IMG电位。结果：在敏感细胞，EGb761(35～350mg／L)可使71．0％的肠系膜下神经节细胞产生膜去极化反应，且在一定范围内呈现剂量效应关系；19．4％的细胞超极化；9．7％的细胞出现先超极化后去极化的双相反应。去极化反应的细胞其膜电阻有降低、增大和不变等多种表现。此去极化反应可被低钙．高镁克氏液部分阻断，但不为胆碱和肾上腺素受体阻断剂所阻断。还发现EGb761可抑制外源性5-HT引起的去极化电位。结论：EGb761对IMG细胞可能通过非胆碱能、非肾上腺素能通路产生易化作用；其机制可能与影响其他递质的释放有关。