人精浆蛋白双向谱型特征及其器官特异性的研究

本文应用双向聚丙烯酰胺凝胶电泳（2D—PAGE）技术分析了60例正常人精浆中的蛋白成分。阐明了人精浆蛋白双向电泳谱型的特征，并将人精浆蛋白双向电泳谱型与其他体液蛋白双向谱型进行了比较，证明人精浆蛋白双向电泳谱型具有器官特异性。本文还用2D－PAGE对不同条件下保存的精斑进行了认定。并将其应用于强奸案的鉴定。     

A comparison of methods used in the UK and Ireland for the extraction and detection of semen on swabs and cloth samples

The recent formation of a United Kingdom and Irish working group, the Body Fluids Forum (BFF), highlighted the need to investigate different working practices prior to any inter-laboratory comparison work and identification of best practice. Various dilutions of semen were seeded onto swabs and cloth samples for each BFF member laboratory to test using their standard techniques. The results showed that the detection of acid phosphatase on swabs is best achieved using direct testing rather than on an extract from the swab. Extraction methods for spermatozoa require a balance to be achieved between using a sufficient volume of water to ensure optimal release and minimal volume to ensure a concentrated extract. PSA tests were investigated and found to be more sensitive than Choline. DNA profiles were obtained from samples in which no spermatozoa had been detected during microscopic examination.     

毓麟2号方对男性不育伴精索静脉曲张患者精液参数及精子DNA碎片指数的影响

目的 观察毓麟2号方对肾虚瘀阻型男性不育伴精索静脉曲张患者精液参数及精子DNA碎片指数(DNA-fragmetation index,DFI)的影响.方法 将118例肾虚瘀阻型男性不育伴精索静脉曲张患者随机分为治疗组和对照组,每组59例.治疗组给予毓麟2号方,对照组给予迈之灵片联合五子衍宗丸.比较两组患者临床综合疗效和...     

高效价鸡抗人精液血清的制备和应用

本文介绍一种制备高效价鸡抗人精血清的方法。给10只2kg 以上的公鸡同时静脉和肌肉注射5～10％混合人精液7～8次,获得特异性很强,效价达50,000倍左右的抗血清。通过实验和检案证明,对由于腐败污染、水浸或水洗而含微量精斑的检材,均能检出。     

Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen

Semenogelin (Sg), a protein originating in the seminal vesicles and a substrate for prostate specific antigen (PSA or p30), is a useful marker for the identification of semen. And detection of Sg has been available commercially in a membrane test recently. PSA is commonly used to detect semen in forensic significant samples taken from sexual assault cases. The strip PSA test has been available commercially from various manufacturers for many years. In this study, we evaluated two immunochromatographic membrane tests, one for Sg and the other for PSA by analyzing human semen, other human bodily fluids/materials including urine, blood, saliva, sweat, breast milk, vaginal secretion and fecal materials, semen from various animals and forensic casework samples. The data demonstrate that both Sg and PSA strip tests provide rapid and sensitive method for identification of seminal plasma. These results show that the immunochromatographic method for Sg detection is useful for the identification of seminal plasma in forensic samples, an alternative to the method for PSA detection.     

A 1-year time course study of human RNA degradation in body fluids under dry and humid environmental conditions

Blood, saliva and semen are some of the forensically most relevant biological stains found at crime scenes. mRNA profiling is a reliable approach for the identification of the origin of an evidentiary trace. A stable set of markers and the knowledge about the effects of RNA degradation under different environmental conditions is necessary for the determination of an unknown biological stain. The aim of this work was to compare RNA degradation for human blood, semen and saliva at three different concentrations during a 1-year time period and exposed to dry and humid conditions. Also, this study addressed the question whether there are relevant differences in the efficiency of two RNA extraction methods.     

Developmental validation of RSID™-Semen: a lateral flow immunochromatographic strip test for the forensic detection of human semen

Abstract: Tests for the identification of semen commonly involve the microscopic visualization of spermatozoa or assays for the presence of seminal markers such as acid phosphatase (AP) or prostate‐specific antigen (PSA). Here, we describe the rapid stain identification kit for the identification of semen (RSID?‐Semen), a lateral flow immunochromatographic strip test that uses two antihuman semenogelin monoclonal antibodies to detect the presence of semenogelin. The RSID?‐Semen strip is specific for human semen, detecting <2.5 nL of semen, and does not cross‐react with other human or nonhuman tissues tested. RSID?‐Semen is more sensitive with certain forensic evidence samples containing mixtures of vaginal secretions and semen than either of the commercially available PSA‐based forensic semen detection tests or tests that measure AP activity that were tested in parallel. The RSID?‐Semen kit also allows sampling a fraction of a questioned stain while retaining the majority of the sample for further processing through short tandem repeat analysis.     

重用生白术和莱菔子治疗功能性便秘68例

目的 观察重用生白术和莱菔子治疗功能性便秘的临床疗效。方法 选择68例功能性便秘患者,以重用生白术和莱菔子的理气润肠通便方药治疗4周,观察便秘改善情况。结果 治疗４周后,患者的排便间隔时间和排便时间较治疗前显著减少（P<0.05）,总有效率为95.58%。结论 重用生白术和莱菔子对功能性便秘具有较好的疗效。     

气相色谱法测定白芥子挥发油中异硫氰酸烯丙酯的含量

目的 采用气相色谱法测定白芥子挥发油中异硫氰酸烯丙酯的含量。方法 采用水蒸气蒸馏法进行挥发油提取,通过气相色谱法测定异硫氰酸烯丙酯含量。色谱柱：RTX-5毛细管柱(30 m×0.53 mm×3.0 μm);进样口温度：230 ℃;检测器温度：260 ℃;载气为高纯氮气, 流速为3 ml/min;进样方式为分流进样,分流比为10〖DK〗∶1;进样量：2 μl。结果 异硫氰酸烯丙酯在10～100 μg/ml范围内线性关系良好（r=0.999 6）;平均回收率为97.64%（RSD=2.39%）。结论 本方法简单、灵敏、重复性好,可用于白芥子挥发油中异硫氰酸烯丙酯的含量测定。     

辽宁汉族人血清类粘蛋白(ORM)型的分布及其在血痕精液(斑)及脑脊液中的检出

本文作者采用PAGIEF结合免疫固定法,调查了辽宁汉族群体(431人)ORM的分布,检出了ORM1型和ORM2型各8种表现型;对不同条件下的血痕标本进行检测,成功地检出了37℃保存2年血痕的ORM1型;首次由脑脊髓液中检出了ORM1型。ORM型的总鉴别机率为0.7043,是进行个人识别和亲子鉴定的良好遗传标记。