鸡传染性支气管炎病毒陕西分离株M蛋白基因的变异及其B细胞表位稳定性

以陕西8株鸡传染性支气管炎病毒(AIBV)分离株M蛋白的基因序列为基础,应用DNAStar软件中的MegAlign模块对其进行了同源性分析;用Kyte-Doolittle方法、Jameson-Wolf方法、Karplus-Schulz方法、Plot-Emini方法和Garnier-Robson方法综合预测了其中4株代表毒株的M蛋白B细胞抗原表位和二级结构。结果,8株AIBV的M蛋白主要在10~40 aa和90~175 aa处存在无规律的点突变,与参考毒株H52、M41、SAIB20、LX和Gray的M蛋白的核苷酸序列和预测的氨基酸序列的同源性分别介于87.4%~99.5%和96.3%~99.5%;分离株间的核苷酸序列和预测的氨基酸序列同源性分别介于91.8%~99.8%和95.4%~100%。4株代表株的M蛋白B细胞优势抗原表位都在159~164 aa和181~193 aa肽段区(这2个区域都包含了无规则卷曲和β-转角结构)或其附近。结果表明,AIBV分离株的M蛋白基因相对保守,只存在无规律的点突变,该变异对AIBV M蛋白B细胞表位的稳定性无影响。     

知识经济的法律调整着力点初探

知识经济的运行 ,是通过知识的产权化、商品化和资本化 ,以实现知识的要素化和产业化的过程 ,与此伴随的还有知识的异化过程。法律应当以知识的产权化、商品化、资本化、要素化、产业化和异化为着力点 ,对知识经济进行调整。     

人类学背景下的语言和言语研究--论语言人类学的研究视野

语言人类学的学科定义以及语言实践、言语行为、指引性和参与性等理论方法的研究视野加深了我们对语言的理解。把语言视作一种交际工具、一种思维模式、一种文化实践 ,即一种行为形式的语言观 ,使得这门边缘学科能够创造性地继续影响它所吸收养分的人文社会学科 ,同时对我们理解语言对于人类的意义有其独特的贡献。语言人类学是人类学背景下的语言和言语研究 ,是把语言作为文化源和言语作为文化实践的研究 ,是语言学的一种人类学阐释。     

索绪尔符号观再评价

语言符号的任意性是贯穿索绪尔语言符号学思想的一条重要原则.任意性理论的提出,并不纯粹是索绪尔本人对符号本质问题深刻反思的结果,而很大程度上得益于对其他许多非语言符号的比较.这是划分两类不同符号系统的一条重要标准.但是,索绪尔过分强调能指的形式侧面在符号结合体中的作用,相反对待号的能指侧面在物质质料构成上的特征,以及这些特征如何有助于区别开不同的符号体系这一问题,却注意不够.符号的解释主体对符号形态的认同,除了符号的所指功能外,实际上对符号的能指侧面的物质质料特征有着很大程度的偏重.这也是符号学的代码理论所要研究的重大问题.     

Post-war Kosovo landscapes in Pristina: discrepancies between language policy and urban reality

This paper focuses on the complex nature of post-war multilingual landscapes in Pristina, the capital of Kosovo, as shaped by the country’s political shift after independence in 2008. We aim to contribute to this sociolinguistically underexplored territory through an examination of the relative predominance and visibility of the capital’s most dominant languages: Albanian, Serbian, and English. Our central aim is to empirically problematize the shared co-officialdom of the Albanian and Serb languages, as put forward in the “Ahtisaari Plan” in 2007 and subsequently adopted in the State Constitution in 2008 and Language Laws in 2006 and 2008. We posit that the multilingual language policies which paint an inclusive, multi-ethnic picture of Pristina do not coincide with its monolingual Albanian reality. In addition to these empirical findings, our second aim is to contribute to the theorization of authorship in the public sphere. With reference to the Pristina context, we problematize the analytical categorization conventionally made between top-down and bottom-up agency and distinguish a third category of semi-official authorship. This third category enables us to examine the dynamic nature of the discrepancy between Kosovo’s language policy and Pristina’s urban linguistic reality in more detail.     

重型犯常见的心理问题及应对策略

重型犯是指犯罪性质恶劣、情节严重以及社会危害性很大的罪犯。用症状自评量表(SCL—90)对重型犯进行了测查,发现入监初期与入监中期服刑人员相比,入监中期服刑人员在躯体化、忧郁、偏执三项因子分方面明显高于入监初期犯人,表明服刑时间较长的罪犯存在更多的心理问题。因此,综合运用各种改造手段对罪犯进行心理和行为矫正,广泛开展心理健康教育,就可以极大地提高我国特殊预防的水平。     

城乡居民收入差异的区域性特点分析

通过分析城乡居民抽样调查资料,建立城乡居民收入差距的回归分析模型,对城乡居民收入差异的区域性特点进行了比较分析。从而为缩小城乡居民收入差距提供了理论参考。     

External Pressures and Internal Dynamics in the Institutionalization of Performance-Based Budgeting: An Endless Process?

The well-known practice of performance-based budgeting (PBB) is a relevant component of the New Public Management (NPM) reform agenda and has become widespread, with varying approaches and results across countries. However, its variation within specific countries has remained largely unexplored. This study analyzes three organizations operating within the same context—three ministries in Italy—to contribute to a new understanding of PBB variation by illustrating why the same PBB practice can or cannot be implemented and internalized similarly across these organizations and thus become (or not) fully institutionalized. The study adopts and enriches the institutional approach by extending beyond isomorphic convergence toward PBB and explaining practice variation, linking the interactions between external pressures and internal dynamics at the organizational level to PBB institutionalization. The empirical analysis shows how a lack of alignment between external pressures and internal dynamics contributes to an unfinished and apparently endless process of institutionalization.     

20世纪乡土小说的创作形态及其新变

从1920年开始,20世纪的中国乡土小说走过了80余年的历程.在不同文化思想的影响下,作家们秉承着不同的创作立场和创作方法,形成了乡土小说的多重创作形态.其中有鲁迅开创的文化批判型,茅盾为中坚的政治功利型,废名和沈从文倡导的文明怀旧型,还有赵树理为代表的乡村代言型.不同的创作形态体现出不同的审美追求和艺术特点,相互之间也构成了复杂的关系.90年代后,乡土小说创作形态呈现出新的发展和变异.     

论侦查讯问中的环境因素

侦查讯问是侦查员与犯罪嫌疑人在特定的环境中以面对面的方式进行的一种特殊的侦查措施,其目的在于获取犯罪嫌疑人的言词证据和线索。特定的环境和面对面的方式是侦查讯问的突出特点。单从环境因素的角度,其可分解为时空环境因素、人际环境因素和语言环境因素。不同的环境因素会对侦查讯问产生不同的影响。