用ELISA法进行AB型体液（斑）与A型、B型混和体液（斑）的血型测定和鉴别

在法医物证检案工作中,经常会遇到ABO型血型的判定,一般通过吸收试验、解离试验、以及混和凝集试验就能够进行分型.但在实际工作中,遇到的检材多数并非单一的体液,而是两种或者两种以上体液的混和斑.特别是强奸案的鉴定,阴道内提取的检材常常是由男性精液和女性阴道分泌物的混合物,要从混和斑中判断各自的血型,目前仍较困难.国内外许多学者都进行了有关的实验,试图

Differentiation of Group ABSe Body Fluids (or Stains)from Mixture of Group ASe and BSe Body Fluids(or Stains)by ELISA

It has been known that blood group substances in body fluids from group AB individuals possess both group A and B activities on the same macromolecules. We applied the results of such studies to differ of group ABSe body fluids (or stains) from mixtures of group ASe and BSe body fluids (or stains) by a sensitive "Sandwich method" enzyme-linked immunosorbent assay (ELISA).The polystyrene ELISA plates were sensitized with monoclonal anti-A or anti-B. A or B substance fixed to plates was quantitatively analysed by the combined use of human anti-A or anti-B and horseradish peroxidase labeled anti-human IgM. The 10000 fold diluted body fluids could be detected by this method. In type AB body fluids, A and B substances were detected with monoclonal anti-A sensitized plate or anti-B sensitized plate. In the mixture of type A and type B body fluids: however, only type A or type B substances were detected with corresponding plate.