针灸督脉防治原发性骨质疏松大鼠的实验研究

目的：建立原发性骨质疏松大鼠模型，探讨针灸督脉早期干预对原发性骨质疏松大鼠骨代谢和激素水平的影响及可能作用机制。方法：选用Wistar大鼠以去卵巢法复制大鼠骨质疏松模型，随机分为假手术组、模型组、针灸督脉组和西药组。针灸督脉组于模型复制后3d开始，观察骨代谢相关指标及激素水平改变特点。结果：模型组血清钙、磷、抗酒石酸酸性磷酸酶(TRAP)、骨密度(BMD)、雌二醇(E2)、降钙素(CT)水平均下降，而碱性磷酸酶(ALP)、甲状旁腺素(PTH)则升高。针灸督脉经3个疗程后对上述指标具有正常化调整作用。结论：针灸督脉早期干预下丘脑-垂体-巨隙轴可以防治原发性骨质疏松症。

Experimental Study on Prevention and Treatment of Primary Osteoporosis in Rats with Acupuncture-moxibustion at Du Channel

Objective:To establish the model of primary osteoporosis in rats. To explore the effect and maybe mechanism of osseous metabolism and hormone level on primary osteoporosis in rats by early intervene with acupuncture moxibustion at Du channel. Method:The model of primary osteoporosis with spay female rats were established.The rats were randomly divided into four groups, namely Pseudooperation group, model group, acupuncture moxibustion group and medication group. The correlation indexes of osseous metabolism and hormone level were observed. Acupuncture moxibustion group were treated after 3 days with spay female rats. Result: The levels of serum calcium, phosphorus, tartrate resistant acid phosphatese(TRAP), bone mineral density(BMD), estradiol(E 2) and calcitonin(CT) of model group were decreased( P <0.05), however alkaline phosphatase(AKP), parathyroid homone(PTH)were increased( P <0.05). Through acupuncture moxibustion at Du channel after three periods of treatment, above indexes were normally modulated. Conclusion: Early intervene on hypothalamic pituitary gonad axes with acupuncture moxibustion at Du channel can prevent and treat primary osteoporosis.