脑络欣通药物血清与胎牛血清诱导大鼠胚胎神经干细胞分化的比较

目的:比较脑络欣通药物血清与胎牛血清诱导大鼠胚胎神经干细胞分化的差异。方法:分别采用脑络欣通药物血清和胎牛血清诱导大鼠胚胎神经干细胞分化,应用相差显微镜和免疫荧光染色对其进行比较观察。结果:脑络欣通药物血清和胎牛血清均可诱导绝大多数神经干细胞分化成神经细胞、星形胶质细胞和少突胶质细胞。此外,脑络欣通药物血清还能在一定程度上促进培养的神经干细胞的增殖。前者诱导干细胞分化的进程比后者要慢,但其分化的神经细胞在细胞形态学上与发育成熟的神经细胞更为接近。结论:脑络欣通药物血清能够诱导神经干细胞分化,并使其分化更加成熟,且在一定程度上促进培养的神经干细胞的增殖。

Comparison Naoluoxintong Serum Supplementation with Fetal Bovine Serum Supplementation in Inducing Differentiation of Rat Embryonic Neural Stem Cells

Objective:To study the difference between Naoluoxintong serum supplementation and fetal bovine serum supplementation in inducing differentiation of rat embryonic neural stem cells.Method:Rat embryonic neural stem cells were differentiated by fetal bovine serum supplementation and Naoluoxintong serum supplementation respectively.Their phenotypic changes were detected via phase contrast microscopy and specific markers of neurocytes(neuron,astrocyte and oligodendrocyte)were detected via immunofluorescence.Result:Both methods induced the majority of neural stem cells to differentiate into neurons,astrocytes or oligodendrocytes.In addition,Naoluoxintong serum supplementation promoted the proliferation of neural stem cells to some extent.Though the differentiative time course of Naoluoxintong serum supplementation was later than that of fetal bovine serum supplementation,the differentiated cells induced by the former morphologically resembled mature neurons to greater extent.Conclusion:Naoluoxintong serum supplementation could not only induce the differentiation of neural stem cells and stimulate the differentiative neurons to develop more mature,but also promote the proliferation of neural stem cells to some extent.