滋阴潜阳方对高血压大鼠淋巴细胞内PKA蛋白表达和cAMP水平的影响

目的:探讨高血压的神经免疫调节机制和滋阴潜阳方对高血压大鼠淋巴细胞内信号转导作用的可能环节.方法:采用免疫组织化学染色法和DP801图像分析系统检测"二肾一夹"高血压大鼠淋巴细胞内蛋白激酶A(PKA)蛋白表达.采用放射免疫法检测环磷酸腺苷(cAMP)水平的改变.结果:模型组大鼠淋巴细胞内PKA蛋白表达和cAMP水平明显增强,滋阴潜阳组对此有抑制作用.结论:滋阴潜阳方抑制淋巴细胞内PKA蛋白表达和cAMP水平可能是其发挥神经免疫调节作用的机制之一.

Effect of Ziyin Qianyang Recipe on Expression of Protein Kinase A and Level of cAMP in Lymphocytes of Rats with Hypertension

Objective:To investigate neuroregulation and immunoregulation mechanisms for hypertension,and explore the potential pathway associated with the effect of Ziyin Qianyang Recipe (ZYQYR) on signal transduction of lymphocytes in rats with hypertension.Methods:The rat hypertension model was established by two-kidney-one-clip method. The expression level of protein kinase A (PKA) in lymphocytes was measured by immunohistochemical staining assay and DP801 Metamorph Imagine Analysis System.The content of cyclic adenosine monophosphate (cAMP) in lymphocytes was assayed by the method of radioimmunity reagent.Results: The expression level of PKA and the level of cAMP increased significantly in model groups compared with those of normal group and sham-operated group.ZYQYR had an inhibitive effect on the increment of PKA and cAMP.Conclusion: It might be one of neuroregulation and immunoregulation mechanisms that ZYQYR inhibits the expression of PKA and the level of cAMP.