| 不同方式标记非放射性探针原位杂交效果评价 |
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| 引用本文： | Liu NG,Zhao ZQ,Gu YJ. 不同方式标记非放射性探针原位杂交效果评价[J]. 法医学杂志, 2002, 18(1): 7-8 |
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| 作者姓名： | Liu NG Zhao ZQ Gu YJ |
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| 作者单位： | 1. 司法部司法部司法鉴定科学技术研究所,上海,200063
2. 复旦大学基础医学院法医学系,上海,200032 |
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| 基金项目： | 国家自然科学基金资助项目(No:39770816) |
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| 摘 要： | 目的比较不同方式标记非放射性探针的原位杂交效果。方法通过两种方法标记地高辛随机引物DNA探针和反意RNA探针,在大鼠损伤模型的皮肤组织上进行原位杂交。结果两种探针均可满足要求,以反意RNA探针在阳性染色深度和背景方面效果更佳。结论RNA探针在杂交效果方面优于DNA探针。
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| 关 键 词： | 原位杂交 地高辛 随机引物探针 反意RNA探针 |
| 文章编号： | 1004-5619(2002)01-0007-02 |
| 修稿时间： | 2001-06-20 |
The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe |
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| Liu Ning-guo,Zhao Zi-qin,Gu Yun-ju. The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe[J]. Journal of Forensic Medicine, 2002, 18(1): 7-8 |
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| Authors: | Liu Ning-guo Zhao Zi-qin Gu Yun-ju |
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| Affiliation: | Institution of Forensic Sciences of Ministry of Justice, Shanghai 200063. liuningguo@163.net |
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| Abstract: | Objective To estimate the effort between antisense RNA probe and random primed DNA probe. Methods The in situ hybridization was conducted on parafin section from wounding model of rat skin. Results Although both probes appeared positive staining, RNA probes was superior to DNA probes in terms of depth of staining and background. Conclusion RNA probe showed more satisfactorily on ISH. |
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| Keywords: | in situ hybridization digoxin antisense RNA probe random primed DNA probe |
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