| 小鼠皮肤切创愈合过程中M_3型乙酰胆碱受体的表达 |
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| 引用本文： | 王涛,官大威,范琰琰,武静静,刘伟伟,赵振宾,于天水,马文翔,胡更奕. 小鼠皮肤切创愈合过程中M_3型乙酰胆碱受体的表达[J]. 法医学杂志, 2010, 26(3): 165-168,172. DOI: 10.3969/j.issn.1004-5619.2010.03.002 |
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| 作者姓名： | 王涛 官大威 范琰琰 武静静 刘伟伟 赵振宾 于天水 马文翔 胡更奕 |
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| 作者单位： | 1. 中国医科大学,法医学院法医病理学教研室,辽宁,沈阳,110001
2. 中国医科大学,基础医学院免疫学教研室,辽宁,沈阳,110001 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 目的 探讨小鼠皮肤切创愈合过程中损伤区M3受体的表达及其随时间的变化规律.方法 应用免疫组织化学染色和Western印迹技术检测小鼠皮肤切创后不同时间段M3受体的变化情况.结果 正常皮肤组织的表皮、毛囊、皮脂腺、汗腺、皮肌层等均表达M3受体.伤后6～12h,损伤区M3受体阳性细胞以多形核粒细胞为主;1～3d,M3受体阳性细胞以单个核细胞和成纤维细胞为主;5～14d,M3受体阳性细胞主要为成纤维细胞.阳性细胞率在伤后6～12h逐渐增高,12h达高峰,1～5d略有下降,但保持较高水平,7d阳性细胞率再次达到峰值,随后逐渐降低.通过Westem印迹法检测显示,各个时间段均有M3受体阳性条带,其中12h和7d为M3受体两个表达高峰.结论 多形核粒细胞、单个核细胞和成纤维细胞均表达M3受体,提示其可能在皮肤切创愈合过程中起重要的作用;M3受体表达的规律性变化可用于损伤时间的推断.
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| 关 键 词： | 法医病理学 创伤和损伤 皮肤 受体,胆碱能 损伤时间 小鼠 |
Expression of M_3 Subtype of Muscarinic Receptor during the Skin Incised Wound Healing in Mice |
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| WANG Tao,GUAN Da-wei,FAN Yan-yan,WU Jing-jing,LIU Wei-wei,ZHAO Zhen-bin,YU Tian-shui,MA Wen-xiang,HU Geng-yi. Expression of M_3 Subtype of Muscarinic Receptor during the Skin Incised Wound Healing in Mice[J]. Journal of Forensic Medicine, 2010, 26(3): 165-168,172. DOI: 10.3969/j.issn.1004-5619.2010.03.002 |
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| Authors: | WANG Tao GUAN Da-wei FAN Yan-yan WU Jing-jing LIU Wei-wei ZHAO Zhen-bin YU Tian-shui MA Wen-xiang HU Geng-yi |
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| Affiliation: | WANG Tao1,GUAN Da-wei1,FAN Yan-yan1,WU Jing-jing2,LIU Wei-wei1,ZHAO Zhen-bin1,YU Tian-shui1,MA Wen-xiang1,HU Geng-yi1(1.Department of Forensic Pathology,School of Forensic Medicine,China Medical University,Shenyang 110001,China,2.Department of Immunology,College of Basic Medical Science,China) |
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| Abstract: | Objective To investigate the expression of M3 subtype of muscarinic receptors(M3R) during the incised wound healing of the skin in mice and the characteristics of its time-dependent.Methods The change of M3R in skin incised wound was detected by immunohistochemical staining and Western blot.Results M3R-positive cells were detected in epidermis,hair follicle,sebaceous glands,sweat glands,dermomuscular layer in normal mouse skin.Expression of M3R was mainly detectable in polymorphonuclear cells(PMNs) in the w... |
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| Keywords: | forensic pathology wounds and injuries skin receptors cholinergic wound age mice |
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