霍山石斛对脾阴虚型慢性萎缩性胃炎大鼠免疫功能的影响

目的 探讨霍山石斛对脾阴虚型慢性萎缩性胃炎大鼠免疫功能的影响。方法 将36只SD大鼠随机分为正常组，模型组，霍山石斛低、中、高剂量组，每组6只，通过灌服N-甲基-N-硝基-亚硝基胍复合温热药（附子、干姜、肉桂）及劳倦法建立脾阴虚型慢性萎缩性胃炎大鼠模型。霍山石斛低、中、高剂量组大鼠分别按0.7、1.4、2.8 g/kg灌胃霍山石斛水悬液，正常组和模型组大鼠灌胃等容积生理盐水，持续灌胃21 d。比较各组大鼠的活动情况，体质量变化，脾脏指数和胸腺指数变化，脾脏组织形态学变化，外周血T淋巴细胞亚群、白细胞计数和分类，血清中免疫球蛋白A（immunoglobulin A， IgA）、免疫球蛋白M(immunoglobulin M， IgM)、免疫球蛋白G（immunoglobulin G，IgG），以及血清和胃黏膜组织中白细胞介素-2（interleukin-2，IL-2）、干扰素-γ（interferon-gamma，IFN-γ）、白细胞介素-4（interleukin-4，IL-4）、白细胞介素-10（interleukin-10，IL-10）水平。结果 各给药组大鼠脾脏指数和胸腺指数均显著上升（P<0.05）；脾脏组织病理状态改善；血清IgA、IgM、IgG水平显著上升（P<0.05）；血清和胃黏膜组织IL-2、TNF-γ水平均有不同程度的升高（P<0.05），IL-4和IL-10水平均有不同程度下降（P<0.05）。此外，霍山石斛可提高大鼠外周血白细胞计数、淋巴细胞计数和中性粒细胞计数（P<0.05）；能上调外周血中CD3+ T淋巴细胞水平、CD4+ T淋巴细胞水平、CD4+/CD8+ ，下调CD8+ T淋巴细胞水平（P<0.05）。结论 霍山石斛可提高脾阴虚型慢性萎缩性胃炎大鼠的免疫功能。

Effect of Dendrobium huoshanense on Immune Function in a Rat Model of Chronic Atrophic Gastritis with Spleen-Yin Deficiency

Objective To investigate effect of Dendrobium huoshanense on immune function in a rat model of chronic atrophic gastritis with spleen-Yin deficiency. Methods A total of 36 Sprague-Dawleyrats were randomly divided into normal group, model group, and low-, middle-, and high-dose Dendrobium huoshanense groups, with 6 rats in each group, and a rat model of chronic atrophy gastritis with spleen-Yin deficiency was established by the administration of N-methyl-N′-nitro-N-nitrosoguanidine combined with Yin-deficiency drugs (Radix Aconiti Lateralis Preparata, dried ginger, and cinnamon) and the fatigue method.The low-, middle-, and high-dose Dendrobium huoshanense groups were given aqueous suspension of Dendrobium huoshanense(0.7, 1.4, and 2.8 g/kg, respectively) by gavage for 21 days, and the normal group and model group were given an equal volume of normal saline by gavage for 21 days.After successful modeling,Dendrobium huoshanense was given by gavage for 21 consecutive days, and the rats in each group were observed in terms of activity, body weight, spleen index and thymus index, spleen histomorphological changes, peripheral blood T lymphocyte subsets, and leukocyte count and classification. ELISA was used to measure the serum levels of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG), as well as the levels of interleukin-2 (IL-2), interferon-γ (IFN-γ), interleukin-4 (IL-4), and interleukin-10 (IL-10) in serum and gastric mucosa. Results All treatment groups had significant increases in spleen index and thymus index, an improvement in the histopathological status of the spleen, and significant increases in the levels of immunoglobulins (IgA, IgM, and IgG) in serum, as well as varying degrees of increases in the levels of cytokines IL-2 and TNF-γ and varying degrees of reductions in the levels of IL-4 and IL-10 in serum and gastric mucosa. In addition,Dendrobium huoshanense improved peripheral blood leukocyte count, absolute value of lymphocytes, and absolute neutrophil count, upregulated peripheral blood CD3+, CD4+, and CD4+/CD8+ ratio, and downregulated the percentage of CD8+ T lymphocytes. Conclusion Dendrobium huoshanense can improve immune function in a rat model of chronic atrophic gastritis with spleen-Yin deficiency.