| 血中碱性药物的高效液相色谱分析 |
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| 引用本文： | 卓先义,吴何坚. 血中碱性药物的高效液相色谱分析[J]. 法医学杂志, 1997, 13(4): 203-204 |
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| 作者姓名： | 卓先义 吴何坚 |
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| 作者单位： | 司法部司法鉴定科学技术研究所!上海200063 |
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| 摘 要： | 利用高效液相色谱法，ODS柱，二极管阵列紫外检测器，测定了血中常见吩噻嗪类、三环类等碱性药物．实验以赛庚啶为内标物，血样经沉淀蛋白后，用固相小柱提取，或直接用乙醚液液提取，以甲醇：水：三乙胺为流动相，考察了血中碱性药物的提取及定性、定量方法．
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| 关 键 词： | 碱性药物 液-固（液）提取 高效液相色谱 |
Determination of basic drugs in blood by RPHPLC |
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| Abstract: | A reveal-phase HPLC method for determination ofphenothiazines and tricyclin antidepressantS in wholeblood was described. In this paper, a model 1090HPLC with DAD and a zorbax ODS column wasused. The mobile phase was methanol: water: tritelyamine (75:24.7:0.3) with PH 7.5. Cyproheptadinewas used as internal standard in this method. Bloodsamples were extracted with the solid-phaseextraction method and the liquid-liquid method. Thismethod is suitable in forensic toxicology analysis forbasic drugs. |
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| Keywords: | HPLC phenothiazines tricyclin forensic toxicology |
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