重复热应激致公兔性腺氧化损伤的研究

为探讨热应激对雄性兔性腺的氧化损伤机制,以体重相近的110日龄雄性新西兰兔为研究对象,每天在固定时间分别以31～34℃和38～40℃进行热应激,并分别在热应激后的第15、30、45及60天处死兔,取样检测性腺组织内总抗氧化酶活性、丙二醛及蛋白质羰基含量。结果显示,随着温度升高,睾丸和附睾组织丙二醛含量增加,总抗氧化酶活性降低,蛋白质羰基含量呈上升趋势,表现出明显的温度-时间效应。表明高热可改变兔睾丸和附睾的正常生理机能,使自由基在组织内累积,引起蛋白质氧化损伤,进而降低繁殖机能。

Effect of reduplicative heat stress on oxidation damage in gonad of male rabbit

To determine the effect of heat stress on oxidative damage in gonad of male rabbits,110 days-old male New Zealand rabbits with the similar body weight were suffered heat stress at 31-34 ℃ and 38-40 ℃ every day in fixed time,samples were taken on day 15,30,45 and 60 after the heat stress respectively,then content of the malondialdehyde(MDA) and protein carbonyl,total antioxidant capacity(T-AOC) in the testis and epididymis were determined.The results showed that with temperature increasing,the MDA content in the testis and epididymis increased,the T-AOC activity decreased and the protein carbonyl content had the rising trend,and displayed significant temperature-time effects.The present study indicated that the high heat stress may change the normal physiological function of the rabbit testicle and epididymis,make free radical accumulate in the organs,cause the oxidation damage of protein,then reduce the reproduction function.