DNA profiling of azoospermic semen samples from vasectomized males by using Y-PLEX 6 amplification kit |
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| Authors: | Shewale Jaiprakash G Sikka Suresh C Schneida Elaine Sinha Sudhir K |
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| Affiliation: | ReliaGene Technologies, Inc., 5525 Mounes St., Suite 101, New Orleans, LA 70123, USA. |
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| Abstract: | Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX 6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1,000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS 19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX 6. |
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