| 山羊痘病毒RPO30基因绿色荧光蛋白质粒的构建及融合表达 |
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| 引用本文： | 赵志荀,吴国华,颜新敏,崔力凡,李健,朱海霞,张强. 山羊痘病毒RPO30基因绿色荧光蛋白质粒的构建及融合表达[J]. 中国兽医科学, 2011, 0(1): 48-52 |
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| 作者姓名： | 赵志荀 吴国华 颜新敏 崔力凡 李健 朱海霞 张强 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室; |
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| 基金项目： | 农业部转基因生物新品种培育重大专项(2009ZX08008-010B); 甘肃省重大科技专项(092NKU032) |
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| 摘 要： | 为了研究针对山羊痘病毒RPO30的siRNAs的干扰效果,构建了RPO30基因与增强型绿色荧光蛋白(EGFP)基因的融合表达质粒,并在BHK-21细胞中表达.通过对RPO30基因进行PCR扩增,克隆入表达载体pEGFP-N1,并进行双酶切及测序鉴定;将阳性重组质粒转染BHK-21细胞,检测绿色荧光蛋白的表达和RPO30...
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| 关 键 词： | 山羊痘病毒 RPO30基因 增强型绿色荧光蛋白 融合表达 |
Construction of recombinant plasmid for fusion expression of goatpox virus RPO30 gene and enhanced green fluorescent protein gene |
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| ZHAO Zhi-xun,WU Guo-hua,YAN Xin-min,CUI Li-fan,LI Jian,ZHU Hai-xia,ZHANG Qiang. Construction of recombinant plasmid for fusion expression of goatpox virus RPO30 gene and enhanced green fluorescent protein gene[J]. Chinese Veterinary Science, 2011, 0(1): 48-52 |
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| Authors: | ZHAO Zhi-xun WU Guo-hua YAN Xin-min CUI Li-fan LI Jian ZHU Hai-xia ZHANG Qiang |
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| Affiliation: | ZHAO Zhi-xun,WU Guo-hua,YAN Xin-min,CUI Li-fan,LI Jian,ZHU Hai-xia,ZHANG Qiang(Key Laboratory of Animal Virology of the Ministry of Agriculture/State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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| Abstract: | The aim of this study was to construct a recombinant plasmid for fusion expression of goatpox virus(GPV) RPO30 gene and enhanced green fluorescent protein(EGFP) gene and express it in BHK-21 cells.The RPO30 gene was amplified from GPV Gulang strain by PCR and inserted into the expression vector pEGFP-N1 containing EGFP gene,and the constructed recombinant plasmid was identified by restriction enzyme digestion analysis and sequencing.BHK-21 cells were transfected with screened positive recombinants and the e... |
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| Keywords: | goatpox virus(GPV) RPO30 gene enhanced green fluorescent protein(EGFP) fusion expression |
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