| 产后子宫内膜炎病牛子宫内支原体的分离与鉴定 |
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| 引用本文： | 郭梦尧,王国卿,张乃生,冯晓声,杨正涛. 产后子宫内膜炎病牛子宫内支原体的分离与鉴定[J]. 中国兽医科学, 2011, 0(1): 36-40 |
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| 作者姓名： | 郭梦尧 王国卿 张乃生 冯晓声 杨正涛 |
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| 作者单位： | 吉林大学畜牧兽医学院;东北大学生物技术研究所; |
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| 基金项目： | 国家“十一五”科技支撑计划项目(3A4090016201) |
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| 摘 要： | 选用产后7～10d的急性脓性子宫内膜炎病牛和健康中国荷斯坦奶牛各20头,通过支原体分离培养、DNA荧光染色法及套式PCR方法,检测了子宫病料中支原体的感染情况,并确定了支原体种类,以探讨支原体感染在奶牛子宫内膜炎中的作用.结果显示,子宫内膜炎病牛子宫的支原体检出率为60%,其中45%发生子宫黏膜细胞感染;而健康组奶牛的...
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| 关 键 词： | 奶牛 子宫内膜炎 解脲支原体 分离 鉴定 |
Isolation and identification of intrauterine Mycoplasma from postpartum dairy cows with endometritis |
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| GUO Meng-yao,WANG Guo-qing,,ZHANG Nai-sheng,FENG Xiao-sheng,YANG Zheng-tao. Isolation and identification of intrauterine Mycoplasma from postpartum dairy cows with endometritis[J]. Chinese Veterinary Science, 2011, 0(1): 36-40 |
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| Authors: | GUO Meng-yao WANG Guo-qing ZHANG Nai-sheng FENG Xiao-sheng YANG Zheng-tao |
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| Affiliation: | GUO Meng-yao1,WANG Guo-qing1,2,ZHANG Nai-sheng1,FENG Xiao-sheng1,YANG Zheng-tao1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.Institute of Biosciences and Biotechnology,Northeastern University,Shenyang 110004,China) |
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| Abstract: | Mycoplasma infection in uterine samples collected respectively from 20 dairy cows with acute blennorrhea endometritis and healthy Chinese Holstein dairy cows in postpartum from day 7 to 10 was determined using Mycoplasma isolation culture,DNA fluorescent staining method and nested PCR to explore the role of Mycoplasma infection in endometritis dairy cows.In result,the positive rate of Mycoplasma in the uterine of endometritis dairy cows was 60%,among them,45% were infected in uterus mucosal cells.While the ... |
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| Keywords: | dairy cow endometritis Mycoplasma urealytium isolation identification |
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