| 大片吸虫ESP对水牛外周血单核/巨噬细胞NO表达的影响 |
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| 引用本文： | 沈前程,林莹,王树艳,张为宇,黄维义,潘玉红,罗华.大片吸虫ESP对水牛外周血单核/巨噬细胞NO表达的影响[J].中国兽医科学,2011(6). |
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| 作者姓名： | 沈前程 林莹 王树艳 张为宇 黄维义 潘玉红 罗华 |
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| 作者单位： | 广西大学动物科学技术学院;广西大学轻工与食品工程学院;广西壮族自治区水牛研究所; |
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| 基金项目： | 广西自然科学基金项目(0832036); 国家自然科学基金项目(30860204) |
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| 摘 要： | 为探讨水牛易感大片吸虫的免疫机理,采用Ficoll-Hypaque密度梯度离心法分离水牛外周血单个核细胞,再经贴壁法纯化单核/巨噬细胞,用Geriss法检测在大片吸虫分泌排泄产物(ESP)或脂多糖(LPS)作用下,水牛外周血单核/巨噬细胞一氧化氮(NO)的表达量。结果显示,大片吸虫ESP或LPS都可单独作用刺激水牛外周血单核/巨噬细胞产生NO。大片吸虫ESP(0.1、1μg/mL)和LPS(0.1μg/mL)联合作用时水牛外周血单核/巨噬细胞产生NO的量低于LPS(0.1μg/mL)单独作用时的量,差异显著(P<0.05)。大片吸虫ESP(10μg/mL)和LPS(0.1μg/mL)联合作用时水牛外周血单核/巨噬细胞产生NO的量与LPS(0.1μg/mL)单独作用时差异不显著(P>0.05)。结果表明,大片吸虫ESP或其中组分能抑制水牛外周血单核/巨噬细胞产生NO的作用,这可能是水牛易感大片吸虫的一个原因。
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| 关 键 词： | 大片吸虫 分泌排泄产物 单核/巨噬细胞 一氧化氮 水牛 |
Effect of excretory and secretary antigen of Fasciola gigantic on expression of NO in buffalo peripheral blood monocytes/macrophages |
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| SHEN Qian-cheng,LIN Ying,WANG Shu-yan,ZHANG Wei-yu,HUANG Wei-yi,PAN Yu-hong,LUO Hua.Effect of excretory and secretary antigen of Fasciola gigantic on expression of NO in buffalo peripheral blood monocytes/macrophages[J].Veterinary Science in China,2011(6). |
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| Authors: | SHEN Qian-cheng LIN Ying WANG Shu-yan ZHANG Wei-yu HUANG Wei-yi PAN Yu-hong LUO Hua |
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| Institution: | SHEN Qian-cheng1,LIN Ying2,WANG Shu-yan1,ZHANG Wei-yu1,HUANG Wei-yi1,PAN Yu-hong3,LUO Hua3(1.College of Animal Science and Technology,Guangxi University,Nanning 530005,China,2.College of Light Industry and Food Engineering,Nanning 530004,3.Guangxi Buffalo Research Institute,Nanning 530001,China) |
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| Abstract: | In order to study the immune mechanism of the susceptibility to Fasciola gigantica in buffalo,mononuclear cells were obtained from buffalo peripheral blood by the method of Ficoll-Hypaque density gradient centrifugation.Macrophages were enriched by adherence.The NO production by excretory and secretory products(ESP) and F.gigantica or lipopolysaccharide(LPS)-stimulated peripheral blood mononuclear cell(PBMC) / macrophage cell(MC)was evaluated by Geriss method.NO was produced by ESP or LPS-stimulated respect... |
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| Keywords: | Fasciola gigantic ESP monocyte/macrophages NO buffalo |
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