| 柔嫩艾美耳球虫DF-1细胞培养体系的建立及应用 |
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| 引用本文： | 姜连连,林矫矫,韩红玉,董辉,赵其平,朱顺海,马卫娇,程军,曾艳波,黄兵.柔嫩艾美耳球虫DF-1细胞培养体系的建立及应用[J].中国兽医科学,2011(6). |
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| 作者姓名： | 姜连连 林矫矫 韩红玉 董辉 赵其平 朱顺海 马卫娇 程军 曾艳波 黄兵 |
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| 作者单位： | 中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费项目(2006JB04) |
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| 摘 要： | 为了建立柔嫩艾美耳球虫的鸡胚成纤维传代细胞(DF-1)培养体系,并应用于柔嫩艾美耳球虫子孢子的细胞入侵活力检测,将CFDA-SE标记的子孢子接入DF-1细胞,利用流式细胞仪测定子孢子的细胞入侵活力。比较了37℃和41℃培养温度下,子孢子对DF-1、MDBK、Vero、BHK、MDCK五种细胞的入侵活力。优化了在子孢子入侵活力检测时,DF-1细胞最佳培养条件。结果表明,柔嫩艾美耳球虫子孢子在DF-1细胞中可发育为第一代裂殖子。在不同培养温度下,子孢子对DF-1细胞的入侵活力均高于其他细胞。优化后的DF-1细胞培养体系的培养程序为:用含100mL/L胎牛血清的DMEM稀释DF-1细胞,以每孔1×105个细胞铺被24孔板,37℃、50mL/L CO2培养24h后,用含50mL/L胎牛血清的DMEM换液后,每孔接入3×105个子孢子,41℃、50mL/L CO2培养8～12h,收集细胞进行检测。
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| 关 键 词： | 柔嫩艾美耳球虫 鸡胚成纤维细胞 入侵活力 CFDA-SE |
Establishment and application of DF-1 cell culture system for the sporozoites of Eimeria tenella |
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| JIANG Lian-lian,LIN Jiao-jiao,HAN Hong-yu,DONG Hui,ZHAO Qi-ping,ZHU Shun-hai,MA Wei-jiao,CHENG Jun,ZENG Yan-bo,HUANG Bing.Establishment and application of DF-1 cell culture system for the sporozoites of Eimeria tenella[J].Veterinary Science in China,2011(6). |
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| Authors: | JIANG Lian-lian LIN Jiao-jiao HAN Hong-yu DONG Hui ZHAO Qi-ping ZHU Shun-hai MA Wei-jiao CHENG Jun ZENG Yan-bo HUANG Bing |
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| Institution: | JIANG Lian-lian,LIN Jiao-jiao,HAN Hong-yu,DONG Hui,ZHAO Qi-ping,ZHU Shun-hai,MA Wei-jiao,CHENG Jun,ZENG Yan-bo,HUANG Bing(Key Laboratory for Animal Parasitology of the Ministry of Agricultury/Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China) |
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| Abstract: | In order to establish a new cell culture system for the sporozoites of Eimeria tenella and apply it to detect the invasion activity of sporozoites,chicken embryo fibroblast cell line(DF-1) was infected with the sporozoites labeled by CFDA-SE and then analyzed by flow cytometry.The invasion activities of sporozoites in MDBK,Vero,BHK and MDCK cells were compared with in DF-1 cells and the culture conditions for DF-1 cells were optimized to obtain the best invasion activity of sporozoites.The results showed th... |
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| Keywords: | Eimeria tenella DF-1 cell invasion activity CFDA-SE |
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