| 鸭疫里氏杆菌地区流行株的分离与外膜蛋白A的变异性分析 |
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| 引用本文： | 毕振威,王永山,杨建朋,欧阳伟,张炜,范红结. 鸭疫里氏杆菌地区流行株的分离与外膜蛋白A的变异性分析[J]. 中国兽医科学, 2011, 0(6) |
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| 作者姓名： | 毕振威 王永山 杨建朋 欧阳伟 张炜 范红结 |
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| 作者单位： | 江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室国家兽用生物制品工程技术研究中心;南京农业大学动物医学院; |
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| 基金项目： | 江苏省自然科学基金资助项目(BK2010471) |
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| 摘 要： | 2010年从安徽省和江苏省境内发病鸭的病理组织中分离到8株鸭疫里氏杆菌(RA),分别被命名为AH1、AH2、AH3、AH4、JS1、JS2、JS3、JS4,其中AH1、JS4为血清1型,AH2、AH3、AH4、JS1、JS2、JS3为血清2型。8株RA的菌体形态、生化特性、PCR鉴定结果相同,但致病力不同,AH1毒力最弱,对鸭的发病致死率仅为16.7%。序列分析结果显示,8株分离菌的外膜蛋白A(OmpA)基因的核苷酸序列同源性为96.9%～100%,氨基酸序列同源性为98.2%～100%。将8株RA与GenBank中登录的其他55株RA的OmpA进行变异性分析,结果其核苷酸序列同源性为89.6%～100%,氨基酸序列同源性为90.2%～100%。从系统发育进化树中可将这63株RA分为4个亚群,不同国家和地区的RA株在亚群中没有显示地域或时间特征,而是呈现出了互相交叉的现象,RA的OmpA差异与血清型、分离时间和分离地点没有必然的联系,本试验近期分离的8个RA株分布在2个亚群中。
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| 关 键 词： | 鸭疫里氏杆菌 分离 外膜蛋白A 变异 |
Isolation of Riemerella anatipestiferfrom Anhui and Jiangsu provinces in 2010 and variation analysis for the outer membrane protein A |
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| BI Zhen-wei,,WANG Yong-shan,YANG Jian-peng,OUYANG Wei,ZHANG Wei,FAN Hong-jie. Isolation of Riemerella anatipestiferfrom Anhui and Jiangsu provinces in 2010 and variation analysis for the outer membrane protein A[J]. Chinese Veterinary Science, 2011, 0(6) |
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| Authors: | BI Zhen-wei WANG Yong-shan YANG Jian-peng OUYANG Wei ZHANG Wei FAN Hong-jie |
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| Affiliation: | BI Zhen-wei1,2,WANG Yong-shan1,YANG Jian-peng1,OUYANG Wei1,ZHANG Wei2,FAN Hong-jie2 (1.Key Laboratory of Animal Diseases Diagnostic and Immunology of the Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products/Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China,2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Eight strains of Riemerella anatipestifer,designated AH1,AH2,AH3,AH4,JS1,JS2,JS3 and JS4,were isolated from Anhui and Jiangsu provinces in 2010.Based on the immunological agglutination tests,AH1 and JS4 represented serotype 1,and AH2,AH3,AH4,JS1,JS2 and JS3 represented serotype 2.The eight isolates were similar in cultural characteristics,bacterial morphology,biochemical properties and the results of PCR amplification,but with different virulence,in which AH1 was the lowest virulent.Sequence analysis of the... |
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| Keywords: | Riemerella anatipestifer(RA) isolation outer membrane protein A variation |
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