| 犬源韦氏巴贝斯虫的分子鉴定和系统发育研究 |
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| 引用本文： | 李高强,张浩吉,张更利,蒲文珺,周庆国,丁巧玲,李金平,李国清. 犬源韦氏巴贝斯虫的分子鉴定和系统发育研究[J]. 中国兽医科学, 2011, 0(3) |
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| 作者姓名： | 李高强 张浩吉 张更利 蒲文珺 周庆国 丁巧玲 李金平 李国清 |
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| 作者单位： | 华南农业大学兽医学院;佛山科学技术学院动物医学系; |
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| 基金项目： | 广东省自然科学基金项目(10152800001000007) |
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| 摘 要： | 无菌采集疑似巴贝斯虫感染血样,进行全血基因组DNA的抽提,用保守引物5-22F和1661R进行巴贝斯虫18SrRNA基因的PCR扩增,扩增产物经克隆、测序后,获得大小为1 677bp的核苷酸序列。序列比对和系统发育分析发现,该序列与韦氏巴贝斯虫(AB083374)18SrRNA基因的相似性达100%,而与犬巴贝斯虫(AY072926)、罗氏巴贝斯虫(L19079)和吉氏巴贝斯虫(AF271081)18SrRNA基因的相似性分别为98.0%,95.2%和93.4%。从分子水平证实了韦氏巴贝斯虫在广东宠物犬的存在,同时也对巴贝斯虫的分类进行了讨论。
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| 关 键 词： | 犬 韦氏巴贝斯虫 18 S rRNA基因 系统进化分析 |
Molecular identification and phylogenetic analysis of Babesia vogeli from dogs |
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| LI Gao-qiang,,ZHANG Hao-ji,ZHANG Geng-li,PU Wen-jun,ZHOU Qing-guo,DING Qiao-ling,LI Jin-ping,LI Guo-qing. Molecular identification and phylogenetic analysis of Babesia vogeli from dogs[J]. Chinese Veterinary Science, 2011, 0(3) |
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| Authors: | LI Gao-qiang ZHANG Hao-ji ZHANG Geng-li PU Wen-jun ZHOU Qing-guo DING Qiao-ling LI Jin-ping LI Guo-qing |
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| Affiliation: | LI Gao-qiang1,2,ZHANG Hao-ji2,ZHANG Geng-li2,PU Wen-jun2,ZHOU Qing-guo2,DING Qiao-ling2,LI Jin-ping1,LI Guo-qing1(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China,2.Department of Veterinary Medicine,Foshan University,Foshan 528231,China) |
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| Abstract: | The 18 S rRNA gene of Babesia was amplified by PCR using a pair of conserved primers(5-22F and 1661R) from canine whole blood genomic DNA extracted from suspected pet dogs in Guangzhou and Foshan.The recombinant plasmids were identified by PCR and the positive clones were sequenced.A 1 677 bp sequence in length was obtained that was identical to the 18 S rRNA gene of Babesia vogeli published previously(AB083374),meanwhile,compared with the 18 S rRNA gene of B.canis(AY072926),B.rossi(L19079) and B.gibsoni(AF... |
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| Keywords: | dog Babesia vogeli 18 S rRNA gene phylogenetic analysis |
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