| 鲫鱼ghrelin基因的克隆和生物信息学分析 |
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| 引用本文： | 周朝伟,林方军,刘遥鹏,胡海平,刘晓凤,张兴栋,刘涛,李洋,李志琼. 鲫鱼ghrelin基因的克隆和生物信息学分析[J]. 中国兽医科学, 2011, 0(2) |
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| 作者姓名： | 周朝伟 林方军 刘遥鹏 胡海平 刘晓凤 张兴栋 刘涛 李洋 李志琼 |
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| 作者单位： | 四川农业大学动物科技学院水产系; |
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| 基金项目： | 四川农业大学双支计划项目(01570702);四川农业大学科研兴趣培养项目(04050637) |
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| 摘 要： | 从鲫鱼的肠道内提取总RNA,运用RT-PCR扩增ghrelin基因并测序,用生物信息学软件预测其结构,并构建系统进化树,探讨了鲫鱼ghrelin基因的生物学功能,为进一步研究该基因对鲫鱼摄食的影响奠定基础。结果显示,获得了长490bp的ghrelin基因(GenBank登录号为HM567312),其序列含有1个312bp的完整开放阅读框,编码103个氨基酸;ghrelin蛋白分子质量为11 519.48u,等电点为5.20,半衰期为1.20h,不稳定系数为31.29,总平均亲水性为0.727,疏水性为-2.900～2.856;1～26位氨基酸可能是信号肽序列;在ghrelin的分子序列中存在44处α螺旋富集区域,8处β折叠区域,集中于成熟肽部分,有43处无规则卷曲。系统进化树分析显示鲫鱼ghrelin基因与金鱼的进化距离最近。
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| 关 键 词： | 鲫鱼 ghrelin基因 克隆 生物信息学分析 |
Cloning and bioinformatics analysis of ghrelin gene in Carassius auratus |
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| ZHOU Chao-wei,LIN Fang-jun,LIU Yao-peng,HU Hai-ping,LIU Xiao-feng,ZHANG Xing-dong,LIU Tao,LI Yang,LI Zhi-qiong. Cloning and bioinformatics analysis of ghrelin gene in Carassius auratus[J]. Chinese Veterinary Science, 2011, 0(2) |
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| Authors: | ZHOU Chao-wei LIN Fang-jun LIU Yao-peng HU Hai-ping LIU Xiao-feng ZHANG Xing-dong LIU Tao LI Yang LI Zhi-qiong |
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| Affiliation: | ZHOU Chao-wei,LIN Fang-jun,LIU Yao-peng,HU Hai-ping,LIU Xiao-feng,ZHANG Xing-dong,LIU Tao,LI Yang,LI Zhi-qiong(Department of Aquaculture,College of Animal Science and Technology,Sichuan Agricultural University,Ya'an 625014,China) |
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| Abstract: | The biological function of ghrelin gene in crucian carp was explored to provide a basis to study effect of the gene on appetite of crucian.The ghrelin gene was amplified from total RNA extracted from the crucian carp intestine by RT-PCR and sequenced and the structure and function of the protein encoded by the gene were investigated.The function of the ghrelin gene-encoded protein was predicted by the bioinformatics software and the phylogenetic tree of ghrelin gene was constructed by CLUSTALW software.In r... |
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| Keywords: | crucian carp ghrelin gene cloning bioinformatics analysis |
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