| 鸭肠炎病毒gB胞浆区基因片段的克隆及原核表达 |
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| 引用本文： | 马波,李洪涛,刘峰源,张扬,刘晓玫,乌伊罕,王君伟. 鸭肠炎病毒gB胞浆区基因片段的克隆及原核表达[J]. 中国兽医科学, 2011, 0(6) |
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| 作者姓名： | 马波 李洪涛 刘峰源 张扬 刘晓玫 乌伊罕 王君伟 |
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| 作者单位： | 东北农业大学动物医学学院;东北农业大学资源与环境学院; |
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| 基金项目： | 东北农业大学博士启动基金项目; 黑龙江省教育厅重大科技项目(10541Z004) |
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| 摘 要： | 为了研究鸭肠炎病毒gB胞浆区的反应原性,以鸭肠炎病毒C-KCE株为模板,扩增了gB胞浆区基因片段,并构建了其原核表达载体pET-32a-gB-N,将其转化至大肠杆菌感受态细胞Rosetta(DE3)pLysS中,经IPTG诱导,用SDS-PAGE及Western-blot检测目的蛋白的表达情况及其反应原性。SDS-PAGE分析表明获得了30ku的融合蛋白,在Western-blot检测中,纯化的融合蛋白可与鸭肠炎病毒阳性血清发生特异性反应,表明其具有良好的反应原性。
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| 关 键 词： | 鸭肠炎病毒 gB胞浆区 反应原性 |
Cloning and prokaryotic expression of cytoplasmic domain of gB gene of duck enteritis virus |
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| MA Boa,LI Hong-taob,LIU Feng-yuana,ZHANG Yanga,LIU Xiao-meia,WU Yi-hana,WANG Jun-weia. Cloning and prokaryotic expression of cytoplasmic domain of gB gene of duck enteritis virus[J]. Chinese Veterinary Science, 2011, 0(6) |
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| Authors: | MA Boa LI Hong-taob LIU Feng-yuana ZHANG Yanga LIU Xiao-meia WU Yi-hana WANG Jun-weia |
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| Affiliation: | MA Boa,LI Hong-taob,LIU Feng-yuana,ZHANG Yanga,LIU Xiao-meia,WU Yi-hana,WANG Jun-weia(a.College of Veterinary Medicine,b.College of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | In order to identify the reactinogenicity of gB cytoplasmic domain of duck enteritis virus(DEV),the interested gene fragment was amplified from the genome of DEV C-KCE strain.The recombinant prokaryotic expression vector pET-32a-gB-N was constructed,and the recombinant plasmid was transformed into Escherichia coli Rosetta(DE3) pLysS.The expressed protein was analyzed by SDS-PAGE,and the reactinogenicity of the purified protein was detected by Western-blot.In result,the expressed fusion protein was 30 ku in ... |
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| Keywords: | duck enteritis virus gB cytoplasmic domain reactinogenicity |
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