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| 免疫荧光及双抗体夹心ELISA在人狂犬病检测中的应用 | |
| 作者姓名： | Sun LJ Xu GL Wang HL Wu J Hu ZH Zhou YW |
| 作者单位： | 1. 华中科技大学同济医学院法医学系,湖北,武汉,430030;中国科学院武汉病毒研究所病毒学国家重点实验室,湖北,武汉,430071 2. 武汉生物制品研究所基因工程室,湖北,武汉,430060 3. 中国科学院武汉病毒研究所病毒学国家重点实验室,湖北,武汉,430071 4. 华中科技大学同济医学院法医学系,湖北,武汉,430030 |
| 基金项目： | 国家自然科学基金资助项目(30570629) |
| 摘 要： | 目的探讨免疫荧光和双抗体夹心ELISA在人狂犬病检测中的应用。方法4例临床诊断狂犬病死亡患者的大脑、小脑、脑干、海马,分别于冰箱和甲醛固定后保存,用上述方法检测。感染狂犬病毒CVS株的鼠脑组织和急性胰腺炎死者脑组织分别作为阳性和阴性对照。结果-70℃保存的人脑和阳性对照狂犬病毒均为阳性,甲醛固定的人脑和阴性对照均为阴性。结论免疫荧光和双抗体夹心ELISA可用于人狂犬病检测,但脑组织要低温保存,不能用甲醛固定。 |
| 关 键 词： | 人狂犬病 法医学鉴定 免疫荧光 双抗体夹心ELISA |
| 文章编号： | 1004-5619(2007)06-0411-03 |
| 修稿时间： | 2007年5月22日 |
Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies |
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| Sun LJ,Xu GL,Wang HL,Wu J,Hu ZH,Zhou YW.Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies[J].Journal of Forensic Medicine,2007,23(6):411-413. | |
| Authors: | Sun Lai-Jing Xu Ge-Lin Wang Hua-Lin Wu Jie Hu Zhi-Hong Zhou Yi-Wu |
| Institution: | Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. sunlaijing@163.com |
| Abstract: | OBJECTIVE: To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies. METHODS: The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 degrees C or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control. RESULTS: Rabies virus was detected in the tissues kept in freezer at -70 degrees C and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control. CONCLUSION: Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution. |
| Keywords: | human rabies forensic identification immunofluorescence Sandwich ELISA with double-antibodies |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |