| 绵羊肺炎支原体免疫蛋白质组学的初步研究 |
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| 引用本文： | 许健,储岳峰,高鹏程,赵萍,贺英,剡根强,逯忠新. 绵羊肺炎支原体免疫蛋白质组学的初步研究[J]. 中国兽医科学, 2012, 0(2): 150-153 |
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| 作者姓名： | 许健 储岳峰 高鹏程 赵萍 贺英 剡根强 逯忠新 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部草食动物疫病重点开放实验室甘肃省生物检测工程技术研究中心;石河子大学动物科技学院 |
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| 基金项目： | 甘肃省科技支撑计划项目(1011NKCA054);甘肃省农业生物技术专项(GNSW-2010-09);国家科技基础性工作专项(2008FY210200);国家现代肉羊产业技术体系项目(CARS-39) |
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| 摘 要： | 利用双向电泳及免疫印迹方法对绵羊肺炎支原体MoGH3-3分离株的抗原蛋白进行筛选,并采用基质辅助激光解吸离子化飞行时间质谱对其进行初步鉴定。结果,共筛选到MoGH3-3株3个主要抗原蛋白点,其中2个被鉴定为延伸因子(elongation factor Tu,EF-Tu),1个为丙酮酸脱氢酶E1-a亚单位(pyruvate dehydrogenase E1-alpha subunit,PDHA)。结果表明,EF-Tu及PDHA是绵羊肺炎支原体主要的抗原蛋白,这为下一步绵羊支原体肺炎基因工程疫苗的研制提供了靶标。
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| 关 键 词： | 绵羊肺炎支原体 免疫印迹 蛋白质组学 |
Preliminary study on immunoproteomics of Mycoplasma ovipneumoniae |
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| XU Jian,CHU Yue-feng,GAO Peng-cheng,ZHAO Ping,HE Ying,YAN Gen-qiang,LU Zhong-xin. Preliminary study on immunoproteomics of Mycoplasma ovipneumoniae[J]. Chinese Veterinary Science, 2012, 0(2): 150-153 |
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| Authors: | XU Jian CHU Yue-feng GAO Peng-cheng ZHAO Ping HE Ying YAN Gen-qiang LU Zhong-xin |
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| Affiliation: | 1 (1.State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Epizootic Diseases of Grazing Animals of the Ministry of Agriculture/Engineering Research Center of Biological Detection of Gansu Province/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China; 2.College of Animal Science and Technology,Shihezi University,Shihezi 832000,China) |
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| Abstract: | The proteins of MoGH3-3 strain of Mycoplasma ovipneumoniae(Mo) were extracted and separated by two-dimensional electrophoresis(2-DE),then subjected to immunoblotting with antiserum of Mo.The screened major immunoreactive proteins were identified by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS)and analyzed with bioinformatics methods. Three major immnunoreactive protein plots were screened with two of them identified as the elongation factor Tu(EF-Tu) and the rest one as the pyruvate dehydrogenase E1-alpha subunit(PDHA). The results showed that EF-Tu and PDHA are the major immnunogenic proteins of Mo. This study provided antigen candidates for the development of novel vaccines against disease caused by Mo. |
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| Keywords: | Mycoplasma ovipneumoniae immunoblotting immunoproteomics |
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