A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material |
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| Authors: | B Budowle A M Gambel |
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| Affiliation: | Forensic Science Research and Training Section, FBI Academy, Quantico, VA. |
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| Abstract: | An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively). |
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