急性吗啡中毒大鼠主要脏器内吗啡分布变化的研究

研究急性吗啡中毒大鼠随给药和死后时间延长 ,主要脏器内吗啡的分布变化规律 ,为吗啡类毒品中毒死亡者尸检取材提供依据。采用免疫组织化学SP技术 ,观察 44只尾静脉注射吗啡的大鼠。从给药后 15min到 5h ,从死后即刻至 48h ,脑、肾、心、肝等脏器内吗啡分布的变化规律。结果表明 ,注射吗啡后短时间内各脏器均有吗啡存在 ,主要分布在某些实质细胞的胞浆内 ,且随时间延长吗啡含量上升 ,达高峰 ( 1h)后逐渐减少或消失。不同组织器官的吗啡含量及其变化速率差异巨大。脑内吗啡出现早 ( 15min) ,消失晚 ( 5h) ,峰值高 ,死后衰减慢 ( 4 8h仍呈强阳性 )。肾脏次之 ,但明显优于心、肝。免疫组化SP技术可作为一种鉴定吗啡类毒品中毒的特异性方法 ,脑、肾是其较理想的检材

Study the distribution patterns of morphine in organs in rats with Acute morphine poisoning

In order to provide insights into sampling in suspected cases of morphine poisoning,the present study was conducted to investigate the distribution patterns of morphine in organs in rats with acute morphine poisoning at different post mortem intervals.Localization and semi quantitation of morphine in the brain,kidney,heart and liver were determined in rats at 15min to 5h after intravenous administration of morphine and at different intervals from 0 to 48h after death using the immunohistochemical SP method and image analysis.The results showed that the morphine could be detected in cytoplasms of certain parenchymal cells in all organs examined short time after delivery of morphine.With the extension of post delivery intervals,the level of morphine inereased,peaked,followed by decrease thereafter.The amount and the distribution patterns of morphine varied greatly with different organs.In the brain,the morphine could be detected earlier than in the heart,kidney and liver.It could detected 15min after the drug administration,with a peak,and disppeared at 5h after drug delivery.It decayed slowly as compared with the heart and lung.The kidney was the second organ in which the morphine was detected early with a high level and decayed slowly.In conclusion,immunohistochemical SP method can be used as a specific technique to detect morphine in organs in suspected cases of morphine poisoning.Both brain and kidney were the candidates for sampling in such case.