弓形虫代谢分泌抗原对猪T细胞亚群的影响 |
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引用本文: | 蔡志杰,王艳华,付宝权,李文卉,芦赟,张燕丽,曹丽艳,张德林. 弓形虫代谢分泌抗原对猪T细胞亚群的影响[J]. 中国兽医科学, 2009, 39(9) |
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作者姓名: | 蔡志杰 王艳华 付宝权 李文卉 芦赟 张燕丽 曹丽艳 张德林 |
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作者单位: | 蔡志杰,芦赟,张燕丽,曹丽艳(中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室,甘肃,兰州,730046;甘肃农业大学,动物医学院,甘肃,兰州,730070);王艳华,付宝权,李文卉,张德林(中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室,甘肃,兰州,730046) |
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基金项目: | 国家"十一五"科技支撑计划项目,公益性行业(农业)科研专项经费项目,甘肃省重大专项科技项目 |
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摘 要: | 为了研究弓形虫代谢分泌抗原对仔猪T细胞亚群及抗体的影响,将仔猪随机分为6组,每组5只,分别为E/SA组、E/SA+CPG(未乳化)组、E/SA+CPG组、E/SA+IL-2组、E/SA+IL-2+CPG组及对照组.分别于免疫前、免疫后第2周、免疫后第4周、感染后第1周及感染后第2周采集仔猪外周血,用流式细胞仪对各试验组外周血中CD4+及CD8+T淋巴细胞亚群的动态变化规律进行检测,用IHA对抗弓形虫抗体水平做检测.结果显示,弓形虫代谢分泌抗原免疫仔猪后第4周,免疫组外周血CD4+、CD8+T淋巴细胞水平和特异性抗体效价与对照组相比均有大幅度升高;感染后第1周,免疫组CD4+T细胞水平及CD4+/CD8+比值较感染前有不同程度降低,感染后第2周,免疫组CD4+T细胞水平及CD4+/CD8+比值上升至免疫后水平,CD8+水平下降,感染后特异性抗体效价进一步升高.表明,弓形虫代谢分泌抗原能够提高仔猪外周血CD4+及CD8+T淋巴细胞水平和特异性抗体水平.
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关 键 词: | 弓形虫 代谢分泌抗原 T细胞亚群 抗体水平 |
Effect of Toxoplasma gondii excreted/secreted antigens on porcine T lymphocyte subgroups |
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Abstract: | To elucidate the effects of Toxoplasma gondii excreted/secreted antigen(E/SA) on piglet's T lymphocyte subgroups and antibodies,piglets were randomly divided into six groups of E/SA, E/SA+CpG(unemulsification),E/SA+ CpG, E/SA+ IL-2, E/SA + IL-2 + CpG groups and the control group. The dynamic changes of the CD4~+ and CD8~+ T lymphocyte subgroups in the piglet's peripheral blood were determined by flow cytometry, and antibody levels were tested by indirect hemagglutination assay. The results showed that on weeks four post-immunization with T. gondii E/SA,the peripheral blood CD4~+ and CD8~+ T lymphocyte levels and specific antibody levels of the immune groups had significantly increased compared with those of the control group. The specific antibody levels further increased on week 1 after inoculation,the CD4~+ T cells levels and CD4~+ /CD8~+ ratio of the immune groups reduced to varying degrees than two weeks post-infection, CD4~+ T cells levels and CD4~ +/ CD8~+ ratio in the immune group increased to immunized levels,CD8~+ levels declined. So the T. gondii E/SA can improve the percentage of the piglet's CD4~+ and CD8~+ T lymphocytes and the specific antibody levels in peripheral blood. |
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Keywords: | Toxoplasma gondii excreted/secreted antigen T lymphocyte subgroup antibody level |
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