酶消化联合强酸消化法的硅藻检验CSCD

目的采用酶消化联合强酸消化法对溺死尸体的器官进行硅藻检验,并评价其应用价值。方法收集40例本地区确证为溺死的尸体,每例提取肺、肝、肾组织及现场水样,分别采用强酸消化法、酶消化联合强酸消化法、酶消化法对组织样本进行硅藻检验,从消化时间、消化能力、硅藻检出率等方面进行比较分析。结果在消化时间、消化能力方面,酶消化联合强酸消化法明显优于酶消化法;在硅藻检出率方面,酶消化联合强酸消化法明显优于强酸消化法。结论酶消化联合强酸消化法结合了强酸消化法和酶消化法的优点,并且操作安全,环境污染小,具有一定的推广和实践价值。

Diatom Detection Using Enzyme Combined with Strong Acid Digestion MethodCSCD

Objective: To detect diatom in the organs of drowners by enzyme combined with strong acid digestion method, and evaluate its application value. Methods: A total of 40 cases which have been identified as drowning in local region were collected. Samples of the lung, liver, kidney, and the water of the scene were also gathered from each case. Strong acid digestion method, enzyme combined with strong acid digestion method, and enzymic digestion method were respectively performed to detect the diatom in the samples. The comparative analysis was made on digestion time, digestive power and detection rate of diatom, etc. Results: Enzyme combined with strong acid digestion method was significantly better than enzymic digestion method on digestion time and digestive power; enzyme combined with strong acid digestion method were obviously superior to strong acid digestion method on the detection rate of diatom. Conclusion Enzyme combined with strong acid digestion method combines the advantages of strong acid digestion method and enzymic digestion method. It has the characters of operation safety with little pollution to environment, which is worthy of further popularization and practice. © 2017 by the Editorial Department of Journal of Forensic Medicine.