PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from BoneCSCD

Objective: To establish a convenient and rapid method for extracting DNA from bone. Methods: Fifteen long bone samples were washed and sterilized. The skeletal fragments were obtained by electric drill, and lysed by PrepFiler Express BTATM lysis buffer. DNA was then manually extracted by silicon microbeads for further analysis. Results: STR genotyping was successfully obtained in 14 out of the 15 samples, and the detection rate was 93.33%. Conclusion: The method for DNA extraction from bone established in present study is convenient, quick, effective, and with a strong applicability, which is worth spreading and applying. © 2017 by the Editorial Department of Journal of Forensic Medicine.