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根据Y小体的出现率确定脏器性别
引用本文:费青,孙景文,孙起富,王建友,裴熙湜.根据Y小体的出现率确定脏器性别[J].中国法医学杂志,1992(4).
作者姓名:费青  孙景文  孙起富  王建友  裴熙湜
作者单位:吉林省公安厅135研究所,吉林省公安厅135研究所,吉林省公安厅135研究所,长春市公安局刑警大队,长春市公安局刑警大队 130062,130062,130062
摘    要:作者用人的心、肝、脾、肺、肾、大脑、小脑制成涂片,用0.5%盐酸阿的平染色,pH5.5磷酸—枸橼酸缓冲液分化,在荧光显微镜下观察细胞核内的Y小体,计数其百分率。共观察了33具尸体的脏器,其中男性22人,女性11人。在男性脏器的细胞核中,Y小体的出现率为24%—80%,均值为48%;女性为0—8%,均值为0.76%;由此可见在两性间Y小体的分布不仅不相重迭,而且男性的下限与女性的上限相距甚远,故用本法进行脏器的性别鉴定是可行的。

关 键 词:脏器  盐酸阿的平  Y小体  荧光显微镜

SEX DETERMINATION OF VARIOUS ORGANSFRAGMENTS BY DETECTING OF Y BODIES
Fei Qing Sun Jingwen Sun Qifu Institute of Jilln Province,ChangChun, Wang Jianyou pei Xishi The Public Security Bureau of ChangChun.SEX DETERMINATION OF VARIOUS ORGANSFRAGMENTS BY DETECTING OF Y BODIES[J].Chinese Journal of Forensic Medicine,1992(4).
Authors:Fei Qing Sun Jingwen Sun Qifu Institute of Jilln Province  ChangChun  Wang Jianyou pei Xishi The Public Security Bureau of ChangChun
Institution:Fei Qing Sun Jingwen Sun Qifu 135 Institute of Jilln Province,ChangChun,130062 Wang Jianyou pei Xishi The Public Security Bureau of ChangChun
Abstract:A Study of the sex determination of human heart,liver,lung,kidney,cerebrum and cerebellum were carried out.smears of organs were made by contacting the slides with cutting surfaces and were then stained with 0.5% Quinacrine 2HC L and differentiated in pH 5.5 macllvain buffer for 5min.The Y bodies were counted under the fluorescence microscope.the detecting rate of Y bodies was ranged from 24%—88% in 22 males,48% in average,while those in females ranged from 0—8%, 0.76% in average.The upper limit in females did not overlap with the low limit in males.It is suggested that the sex determination with this method is reliable.
Keywords:Human organ Quinacrine 2HCL Y body Fluorescence microscope
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