DNA指纹图在法医学鉴定中应用的研究(Ⅱ)——应用‘Myo’小卫星探针进行血斑、精斑、个体组织的个体认定

应用‘Myo’小卫星 DNA 探针,Southern 印迹杂交技术,对血斑、精斑、同一个体不同组织进行 DNA 指纹图分析,均获得清晰的图谱。同一个体的血斑与血液、精斑与精液以及不同的组织其 DNA 指纹图谱完全相同。可以根据斑痕或组织与嫌疑个体的血液或某一组织 DNA 的指纹图谱比对以做出同一认定。50μl 血液量的血斑、5μl 精液量的精斑可以获得清晰易辨的指纹图谱。五年的精斑、两年的血斑亦可做出与同源个体新鲜精液、血液完全一致的 DNA 指纹图谱。对杀人、强奸杀人、碎尸等不同案件的血痕、精斑、不同组织碎块进行了 DNA 指纹图检验,均做出了正确的个体认定。本方法的应用为我国法医物证检验提供了新的分析手段,使个体认定得以实现。

THE PRACTICAL USE OF DNA FINGERPRINTS FOR PERSONAL IDENTIFICATION IN CRIMINAL CASES

Analysis of DNA fingerprint patterns of bloodstains,semenstains,andof different tissues from the same individual,using‘Myo’minisatellite DNAprobe,Southern blotting hybridization technique was carried out.DNAfingerprint patterns from the bloodstains,blood,semenstains,semen,anddifferent tissues of the same individual were all the same.Personal identfi-cation could be achieved by comparing the DNA fingerprint patterns of blood-stains or tissues left on the weapon,scene or the suspect's cloth withthe DNA fingerprint patterns of blood or tissues from victim or the suspect.Distinct fingerprint patterns can be obtained from 50 μl fresh blood or the blood-stains containing equivalent amount of blood,and also from 5 μl fresh semenor the equivalent stain.The DNA fingerprint patterns can also be obtainedfrom the 5 year old semenstains,2 year old bloodstains.DNA fingerprintanalysis of bloodstains,sementains and tissues in cases of homicide,anddismembered body was conducted.Good results were obtained.