Nondestructive Methods for Recovery of Biological Material from Human Teeth for DNA Extraction |
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| Authors: | Montserrat Hervella Ph.D. Maitane G. Iñiguez M.Sc. Neskuts Izagirre Ph.D. Alberto Anta Ph.D. Concepción de‐la‐Rúa Ph.D. |
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| Affiliation: | 1. Faculty of Science and Technology, Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, Leioa, Spain;2. Faculty of Medicine and Odontology, Department of Stomatology I, University of the Basque Country UPV/EHU, Leioa, Spain |
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| Abstract: | The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces. |
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| Keywords: | forensic science human teeth ancient DNA DNA recovery mitochondrial DNA nuclear DNA |
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