人工感染PRRSV仔猪肺和子宫细胞凋亡的电镜观察

应用透射电子显微镜对仔猪感染猪生殖与呼吸综合征病毒(PRRSV)后不同时期肺和子宫凋亡细胞的超微形态结构进行了观察。结果表明,PRRSV可诱导宿主肺和子宫发生典型的细胞凋亡,表现为细胞的超微形态结构随着病毒感染进程出现不同的特征性变化,早期(感染后第8 h至第3 d)凋亡细胞多表现为细胞体积缩小,细胞质密度增强,染色质浓缩,核仁解体,内质网扩张;中期(感染后第5 d至第9 d)凋亡细胞体积显著缩小,细胞膜突起,线粒体增多,有凋亡小体形成;晚期(感染后第10 d以后)凋亡细胞形态多表现为凋亡小体降解和消失,少数凋亡细胞在凋亡晚期表现为坏死细胞。肺细胞凋亡主要发生于肺巨噬细胞和肺泡上皮细胞,子宫细胞凋亡主要发生于子宫内膜细胞和内膜腺细胞。

Observation on apoptosis in lung and uterus of piglets infected with PRRSV by electron microscope

The ultrastructural changes of apoptosis of lungs and uteri were observed by (electron) microscope and analyzed after piglets had been infected with porcine reproductive and respiratory syndrome virus (PRRSV). The results indicated that the typical apoptosis was induced by PRRSV in lungs and uteri. The (ultrastructural) changes showed different characterizations at different stages of the infection. During the early stage from 8th h to day 3 post-infection,the apoptosis cells shrank, the cytoplasm concentrated, the chromatin condensed, the kernel disaggregated and the endoplasmic reticulum dilated. During the middle stage from day 5 to day 9 post-infection,the apoptosis cells evidently shrank, the cell membranes protu- berated, the plastosome manifolded, and apoptosis bodies were found. During the last stage from day 10 upwards post-infection, the apoptosis bodies degenerated and disappeared, and no inflammation occurred. Few apoptosis cells were necrotic. The apoptosis of lungs mostly occurred in the macrophage or in the (epithelial) cells. The apoptosis of uteri mainly occurred in the endomembrane cells or the endomembrane gland cells.