| HRP标记探针MVR分型法在法医学上的应用 |
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| 作者姓名： | Zheng X Ye J Ni J Li B |
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| 作者单位： | 公安部物证鉴定中心!北京,100038,公安部物证鉴定中心!北京,100038,公安部物证鉴定中心!北京,100038,公安部物证鉴定中心!北京,100038 |
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| 摘 要： | 采用辣根酶标记寡核管酸探针数字编码小卫星MS可变重复序列（HRP标记探针MVR方法）,检验微量生物材料。结果表明,相当于5μl血液的血痕、单根有毛囊的毛发以及相当于4μl唾液的斑迹均获得了清晰易读的MVR图谱,灵敏度达1ng。将此方法应用于检案实践取得了较好的效果。MVR－PCR技术在法医学个人识别和亲子鉴定方面极有应用前景。
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| 关 键 词： | MVR－PCR 辣根酶数字编码 DNA分型 个人认定 |
Application of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probes |
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| Zheng X,Ye J,Ni J,Li B.Application of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probes[J].Journal of Forensic Medicine,1998,14(1):14-15,17. |
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| Authors: | Zheng X Ye J Ni J Li B |
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| Institution: | Institute of Forensic Science, P.R. China, Beijing 100038. |
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| Abstract: | The pulPose of this study is to detect small amount ofbiological materials using the method of MVR-PCR atDIS8 locus with HRP-labeled MS32 oligonucleotidesprobe. The results showed that the MVR-PCR patterns,obtained from trace blood stain (equal to 5 ul blood),trace saliva stain (equal to 4 ul saliva) or a hair root, canbe read clearly and easily. The sensitivity of the methodis 1 ng DNA. The applications of the method in personalidentification were discussed. |
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| Keywords: | D1S8 locus MVR-PCR HRP-labeled probe |
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