三江白猪Hal基因型及其血液生化指标的测定

随机选取216头三江白猪,提取基因组DNA进行聚合酶链式反应,获得了氟烷基因(Hal)特异片段,测得其显性等位基因HalN的基因频率为92.13%,隐性等位基因Haln的基因频率为7.87%。然后对不同氟烷基因型三江白猪的14项血液生化指标进行了测定,结果表明:HalNHalN基因型三江白猪的葡萄糖含量、丙氨酸氨基转移酶活性与HalNHaln、HalnHaln基因型的相应指标之间差异显著(P<0.05),HalNHaln与HalnHaln两指标之间差异不显著(P>0.05)。3种基因型之间乳酸脱氢酶、天门冬氨酸氨基转移酶、胆碱酯酶、碱性磷酸酶活性以及总胆固醇、总胆红素、总蛋白、白蛋白、钙、磷、甘油三酯、肌酐含量差异显著(P<0.05)。

Detection of Hal genotypes and blood biochemical parameters in Sanjiang White pigs

Hal gene was amplified by PCR from genomic DNA of 216 randomly-selected Sanjiang White pigs and Hal~(N) and Hal~(n) gene frequencies were detected to be 92.13% and 7.87% respectively.Afterwards 14 blood biochemical parameters were detected in different Hal genotypes of Sanjiang White pigs.Results were as follows.Difference of glucose level and alanine amino transferase activity between Hal~(N)Hal~(N) and Hal~(n)Hal~(n) Sanjiang White pigs was significant(P<0.05),and between Hal~(N)Hal~(n) and Hal~(n)Hal~(n) was not significant(P>0.05).Difference of lactate dehydrogenase,aspartate amino transferase,cholinesterase and alkaline phosphatase activities and total cholesterol,total bilirubin, total proteins,albumin,calcium,phosphorus,triglyceride and creatinine levels among Hal~(N)Hal~(N),Hal~(N)Hal~(n) and Hal~(n)Hal~(n) pigs was significant(P<0.05).