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利用Identifiler分型系统推断同胞关系
引用本文:郭燕霞,康艳荣,李万水,陈松,张国臣,刘开会,郭燕东,李嘉丽,郭红玲,郝金萍,刘路,叶健,徐小玉,欧元,张建,林小健,王华,翟亚森,米瑞华. 利用Identifiler分型系统推断同胞关系[J]. 中国法医学杂志, 2009, 24(6): 368-371
作者姓名:郭燕霞  康艳荣  李万水  陈松  张国臣  刘开会  郭燕东  李嘉丽  郭红玲  郝金萍  刘路  叶健  徐小玉  欧元  张建  林小健  王华  翟亚森  米瑞华
作者单位:1. 公安部物证鉴定中心,北京,100038
2. 广州医学院港湾医院,广东广州,510700
3. 遵义市公安局,贵州遵义,563000
4. 宁夏回族自治区公安厅,宁夏银川,750001
5. 黔南州公安局,贵州都匀,558000
6. 新疆维吾尔自治区公安厅物证鉴定中心,新疆乌鲁木齐,830000
摘    要:目的探讨自主开发的同胞关系鉴定自动分析软件(ASI)对Identifiler分型系统进行同胞关系鉴定的可行性。方法应用本课题组所开发的软件ASI,对151对同胞及31 224对人工模拟无关个体进行Identifiler系统的15个常染色体STR基因座分型进行分析,计算亲权指数(PI)、同胞关系概率(WFS)和等位基因匹配情况,所获数据进行统计分析,自动计算排序。结果当WFS大于99.999%时,同胞个体占39.07%,无关个体占0%,两组具有显著差异,可以推断两个体同胞关系。当WFS介于1%~99.999%范围内,同胞个体和无关个体有部分重叠,同胞个体占60.93%,无关个体占21.3%,两者具有一定差异,可以通过增加检测STR基因座,再结合案情加作Y-STR、m tDNA检测,以推断两个体是否具有同胞关系。当WFS小于1%时,同胞个体占0%,无关个体占78.7%,可以推断两个体不具有同胞关系。个体间等位基因匹配结果表明:在检测Identifiler体系15个STR基因座时,当两个体常染色体STR基因座的全相同数目≥5时,或全不同数目≤1时,提示为同胞关系;当两个体全不同数目≥6时,或全相同数目≤1时,提示为无关个体,以此作为预测有无同胞关系的界值。结论Identifiler系统及同胞关系鉴定自动分析软件ASI可用于推断同胞关系。

关 键 词:法医物证学  常染色体  同胞鉴定  同胞关系鉴定自动分析软件ASI  Identifiler体系

Research siblings identification by Identifiler system and automatic STR genetic
GUO Yanxia,KANG Yanrong,LI Wanshui,CHEN Song,ZHANG Guochen,LIU Kaihui,GUO Yandong,LI Jiali,GUO Hongling,HAO Jinping,LIU Lu,YE Jian,XU Xiaoyu,OU Yuan,ZHANG Jian,LIN Xiaojian,WANG Hua,ZHAI Yashen,MI Ruihua. Research siblings identification by Identifiler system and automatic STR genetic[J]. Chinese Journal of Forensic Medicine, 2009, 24(6): 368-371
Authors:GUO Yanxia  KANG Yanrong  LI Wanshui  CHEN Song  ZHANG Guochen  LIU Kaihui  GUO Yandong  LI Jiali  GUO Hongling  HAO Jinping  LIU Lu  YE Jian  XU Xiaoyu  OU Yuan  ZHANG Jian  LIN Xiaojian  WANG Hua  ZHAI Yashen  MI Ruihua
Affiliation:GUO Yanxia1,KANG Yanrong1,LI Wanshui1,CHEN Song1,ZHANG Guochen1,LIU Kaihui1,GUO Yandong2,LI Jiali2,GUO Hongling1,HAO Jinping1,LIU Lu1,YE Jian1,XU Xiaoyu1,OU Yuan1,ZHANG Jian1,LIN Xiaojian3,WANG Hua4,ZHAI Yashen5,MI Ruihua6/1.Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China,2.Gangwan Hospital,Guangzhou Medical University,Guangzhou 510700,3.Public Security Bureau of Zunyi,Guizhou 563000,4.Institute of Public Security of Ningxia Province,Yinchuan 750001,5.Public ...
Abstract:Objective To evaluate the probability of siblings identification in Identifiler system by using the software of automatic analysis.Methods Using the software of automatic analysis in siblings identification,STR genetic typing of 151 pairs of full siblings and 31224 pairs of unrelated individuals from manual simulation were analyzed in 15 STR loci of Identifiler system.Results Kin probability(WFS)of 39.07% full siblings were more than 99.999% while WFS of unrelated individual pairs were 0%.WFS of 60.93% full...
Keywords:forensic biological evidence  auto-some  full sibling identify  automatic analysis software  identifiler system
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