| 实验性大鼠脑震荡后c-myc癌基因的表达 |
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| 作者姓名： | Fang WH Wang DL Wang F |
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| 作者单位： | 1. 皖南医学院法医系,安徽,芜湖,241001
2. 芜湖县公安局,安徽,芜湖,241100 |
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| 摘 要： | 目的探讨癌基因c-myc的表达与脑震荡性损伤的关系。方法60只实验大鼠随机分为脑震荡组和对照组。用免疫组织化学SABC法观察大鼠脑震荡后不同时间段脑内c-myc蛋白表达的规律。结果对照组大鼠未见c-myc蛋白的表达;脑震荡组损伤后20min可在神经细胞内观察到c-myc蛋白的表达,随着损伤后经过时间的延长,c-myc蛋白阳性细胞数及阳性表达范围逐渐扩大,损伤后8h表达达高峰,随后阳性细胞数和表达范围逐渐缩小。结论c-myc原癌基因的检测可能成为诊断脑震荡的一个敏感指标。
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| 关 键 词： | 脑震荡 c-myc蛋白 表达 免疫组织化学 |
| 文章编号： | 1004-5619(2006)05-0333-02 |
| 修稿时间： | 2006年3月14日 |
Expression of c-myc protein on rats' brains after brain concussion |
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| Fang WH,Wang DL,Wang F.Expression of c-myc protein on rats' brains after brain concussion[J].Journal of Forensic Medicine,2006,22(5):333-334,F0004. |
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| Authors: | Fang Wei-Hua Wang Dong-Liang Wang Feng |
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| Institution: | Department of forensic medicine, Wannan medical college, Wuhu 241001, China. weihua_3110@yahoo.com.cn |
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| Abstract: | OBJECTIVE: To study the changes of expression of c-myc protein on rats' brains after brain concussion. METHODS: sixty rats were randomly divided into brain concussion groups and control group. The expression of c-myc protein was microscopically observed by immunohistochemical method. RESULTS: No expression of c-myc protein in control group were observed. However, positive expression of c-myc protein in some neurons was seen at 20 min after brain concussion, and reach to the peak at 8h after brain concussion and then decreased gradually. CONCLUSION: These findings suggest that the detection of c-myc protein could be an index of diagnosis of brain concussion. |
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| Keywords: | brain concussion c-myc protein expression immunohistochemistry |
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