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| 仔猪大肠杆菌病K88-K99-987P-F41四价亚单位疫苗制苗株的生物学特性 | |
| 引用本文： | 袁万哲,何孔旺,陆承平. 仔猪大肠杆菌病K88-K99-987P-F41四价亚单位疫苗制苗株的生物学特性[J]. 中国兽医科学, 2005, 35(1): 10-13 |
| 作者姓名： | 袁万哲 何孔旺 陆承平 |
| 作者单位： | 1. 河北农业大学,动物科技学院,河北,保定,071001;江苏省农业科学院,农业部畜禽疫病诊断重点开放实验室,江苏,南京,210014 2. 江苏省农业科学院,农业部畜禽疫病诊断重点开放实验室,江苏,南京,210014 3. 南京农业大学,动物医学院,江苏,南京,210095 |
| 基金项目： | 江苏省十五农业攻关(三药)项目(BE2001604) |
| 摘 要： | 对仔猪大肠杆菌病K88 ( 为黏附素阳性)、K99 、987P 、F41 制苗株的血凝性、传代稳定性等生物学特性进行了研究。结果表明,K88 株不能凝集绵羊红细胞,但能凝集鸡、猪、豚鼠、兔红细胞,以鸡红细胞凝集价最高;K99 株不能凝集兔红细胞,但能凝集鸡、猪、豚鼠、绵羊红细胞,以绵羊红细胞凝集价最高;F41 株对这5种红细胞均能凝集,以绵羊红细胞凝集价最高;而987P 株对这5种红细胞均不能凝集。传代结果表明,K88 株使用限定代次可达22代,K99 、987P 、F41 至少可达42代。 |
| 关 键 词： | 产肠毒素性大肠埃希氏菌 黏附素 甘露糖抵抗血凝试验 传代 |
| 文章编号： | 1000-6419(2005)01-0010-04 |
| 修稿时间： | 2004-09-15 |
Study on biological properties K88-K99-987P-F41 tetravalent subunit vaccine against piglet colibacillosis |
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| YUAN Wan-zhe. Study on biological properties K88-K99-987P-F41 tetravalent subunit vaccine against piglet colibacillosis[J]. Chinese Veterinary Science, 2005, 35(1): 10-13 | |
| Authors: | YUAN Wan-zhe |
| Affiliation: | YUAN Wan-zhe~ |
| Abstract: | K_(88)~( ),K_(99)~( ),987P~( )and F_(41)~( )strains were studied with respect to their some biological characteristics,including hemagglutination and passage stability. The mannose resistant hemagglutination test(MRHA) showed that K_(88)~( )strain could agglutinate erythrocytes of chicken,pig,guinea pig and rabbit,but not of sheep.For all sources of erythrocytes examined,chicken erythrocytes had the highest agglutination titers. K_(99)~( ) strain could do erythrocytes of chicken,pig,guinea pig and sheep,but not of rabbit.The sheep erythrocytes could receive the highestagglutination titers,which was the same to F_(41)~( ).F_(41)~( )strain could do those all erythrocytes,but 987P~( )not.Passage results showed K_(88) strain could passageto 22nd passage,K_(99)~( ),987P~( )and F_(41)~( ) strains could do to 42nd passage at least. |
| Keywords: | enterotoxigenic Escherichia coli adhesin MRHA passage |
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