| 无色孔雀石绿免疫原的合成与鉴定 |
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| 引用本文： | 王权,李健,郭德华,龚鹏飞. 无色孔雀石绿免疫原的合成与鉴定[J]. 中国兽医科学, 2007, 37(6): 534-538 |
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| 作者姓名： | 王权 李健 郭德华 龚鹏飞 |
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| 作者单位： | 1. 中国农业科学院,上海兽医研究所,兽医公共卫生研究室,农业部动物寄生虫学重点开放实验室,上海,200232
2. 上海出入境检验检疫局,上海,200135 |
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| 基金项目： | 上海市科技兴农科技攻关项目 |
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| 摘 要： | 用化学方法合成了硝基无色孔雀石绿(NO2-LMG),还原为氨基无色孔雀石绿(NH2-LMG),经红外光谱扫描鉴定NH2-LMG为所需化合物。氨基无色孔雀石绿经重氮法处理后与兔血清白蛋白(RSA)载体蛋白相连,通过紫外分光光谱鉴定,偶联产物与其前体物相比吸收峰发生了飘移,说明偶联成功。通过动物免疫试验和ELISA抗体特异性试验对该人工合成抗原免疫原性和与无色孔雀石绿反应原性的鉴定,表明成功合成了无色孔雀石绿免疫原。
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| 关 键 词： | 无色孔雀石绿 免疫原 合成 |
| 文章编号： | 1673-4696(2007)06-0534-05 |
| 修稿时间： | 2006-11-13 |
Synthesis and identification of immunogen for leucomalachite green |
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| WANG Quan,LI Jian,GUO De-hua,GONG Peng-fei. Synthesis and identification of immunogen for leucomalachite green[J]. Chinese Veterinary Science, 2007, 37(6): 534-538 |
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| Authors: | WANG Quan LI Jian GUO De-hua GONG Peng-fei |
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| Abstract: | NO2-LMG was synthesized by chemical method and reduced to NH2-LMG.Then,NH2-LMG was identified by the infrared spectrometry(IRS).NH2LMG treated with diazotization was linked with rabbit serum albumin(RSA),which was proved using the UV spectrophotometer scanning absorption peak floating.The immunogenicity and reactogenicity for the LMG of artificial antigen were examined using animal immunity test and ELISA test.The result showed that the LMG-RSA immunogen was successfully obtained. |
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| Keywords: | leucomalachite green immunogen synthesis |
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