| 大鼠心肌缺血再灌流损伤的免疫组化研究 |
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| 引用本文： | 傅晨钟,宋一璇,薛冠华,祝家镇,毕启明. 大鼠心肌缺血再灌流损伤的免疫组化研究[J]. 中国法医学杂志, 1993, 0(4) |
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| 作者姓名： | 傅晨钟 宋一璇 薛冠华 祝家镇 毕启明 |
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| 作者单位： | 中山医科大学法医病理学教研室，中山医科大学法医病理学教研室，广东省老年病学研究所，中山医科大学法医病理学教研室，中山医科大学法医病理学教研室 (广州510089)，(广州510089)，(广州510089)，(广州510089) |
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| 摘 要： | 用16只 SD 大白鼠建立缺血再灌流心肌损伤模型,其中8只预先静脉注射吗啡以抗心律失常。此外,再用8只大鼠开胸作正常对照。其心脏标本进行 HE 染色及抗肌动蛋白单克隆抗体(HHF_(35))免疫组化观察:对照组心肌呈均匀一致的棕褐色染色;注射吗啡后再灌流组心肌呈小灶性分布的 HHF_(35)染色缺染区;未注射吗啡组心肌缺染呈广泛大面积分布。结果表明,HHF_(35)免疫组化法对显示实验性心肌缺血再灌流损伤有重要价值,且心肌缺血再灌流损伤程度与心律失常有关。
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| 关 键 词： | 心肌缺血再灌流损伤 心律失常 抗肌动蛋白单克隆抗体(HHF_(35)) 免疫组化 |
IMMUNOCYTOCHEMICAL STUDY WITH ANTI-MUSCLE ACTIN ANTIBODY(HHF_(35))ON MYOCARDIAL ISCHEMIA AND REPERFUSION INJURY IN RATS |
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| Fu chenzhong Song Yixuan Xue Guanhua Zhu Jiazhen Bi Qiming. IMMUNOCYTOCHEMICAL STUDY WITH ANTI-MUSCLE ACTIN ANTIBODY(HHF_(35))ON MYOCARDIAL ISCHEMIA AND REPERFUSION INJURY IN RATS[J]. Chinese Journal of Forensic Medicine, 1993, 0(4) |
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| Authors: | Fu chenzhong Song Yixuan Xue Guanhua Zhu Jiazhen Bi Qiming |
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| Affiliation: | Fu chenzhong Song Yixuan Xue Guanhua Zhu Jiazhen Bi Qiming Department of Forensic Pathology,Sun Yat Sen University of Medical Sciences,Guangzhou,510089 |
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| Abstract: | Experimental studies on the myocardial ischemia and reperfusion injury in 16 anaethetized SDrats,of which,8 animals were pretreated with morphine(5 mg/kg,i.p.)for preventing of arrhyth-mias,were studied immunocytochemically with anti-muscle actin specific monoclonal antibody (HHF_(35)),8 shan-operated rats were used as control.With HHF_(35) ABC immunocytochemical method,the area of myocardial ischemia and reperfusion injury(without morphine)showed decrease or ab-sence of staining,large areas of staining loss were also seen.In the group with morphine,only smallfoci of staining absence were shown.The myocardium in control animals showed evenly positive stain-ing.No change were seen with HE staining in all groups.The results obtained with HHF_(35) stainingsupport its important value in studying on myocardic reperfusion injury,and indicated that the degreeof myocardic damage may be relative to the arrhythmias in myocardial reperfusion injury. |
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| Keywords: | Myocardium ischemia reperfusion injury Arrhythmias Anti-muscle actin antibody Immunocytochemistry |
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