| 体外DNA扩增技术鉴定血痕性别三例报告 |
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| 引用本文： | 吴梅筠,孙光云. 体外DNA扩增技术鉴定血痕性别三例报告[J]. 中国法医学杂志, 1991, 0(2) |
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| 作者姓名： | 吴梅筠 孙光云 |
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| 作者单位： | 华西医科大学法医系血清学教研室，华西医科大学法医系血清学教研室 成都 610041，成都 610041 |
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| 摘 要： | 本文报道用体外DNA扩增技术对3例刑事案中的人血痕标本进行性别鉴定。其方法是从血痕标本中微量抽提DNA,用蛋白酶K进行消化。然后用两组引物Y1.1与Y1.2和Alu9.1与Alu9.2及国产FD耐热DNA聚合酶进行聚合酶链反(PCR)扩增DNA,电泳分析Y及Alu重复序列,从而判断血痕的性别。
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| 关 键 词： | 人类干血痕 性别鉴定 DNA扩增 聚合酶链反应(PCR) |
THE APPLICATION OF DNA AMPLIFICATION TECHNIQUE IN VITRO IN FORENSIC MEDICNE PRACTICE |
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| Wu Meiyun Sun Guangyun. THE APPLICATION OF DNA AMPLIFICATION TECHNIQUE IN VITRO IN FORENSIC MEDICNE PRACTICE[J]. Chinese Journal of Forensic Medicine, 1991, 0(2) |
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| Authors: | Wu Meiyun Sun Guangyun |
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| Affiliation: | Wu Meiyun Sun Guangyun Department of Forensic Serology West China University of Medical Sciences,Chengdu 610041 |
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| Abstract: | This paper reports the determination of sex of human blood stains in threecriminal cases by the DNA amplification technique.The results fit the cases de-tails and gave the scientific evidence for the justice.The blood stains were dige-sted with proteinase K.The protein was extracted with the phenol-chloroform.The DNA was precipitated with NaCl and ethyl alcohol.Amplification of DNAwas carried out using two pairs of primer Y1.1 Y1.2 and Alu9.1 Alug.2 and heatstable FD polymerase.The amplified products were subjected to agarose gel(con-taining ethidium bromide)electrophoresis.Sex of blood stains was determinedaccording to the amplified products.PCR technique is rapid to perform,sensitiveand simple.No special equipments and isotope labelled probe are required. |
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| Keywords: | Human blood stain Sex identification DNA amplification Polymerase chain reaction(PCR) |
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