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A method to increase the volume of sample applied to isoelectric focusing gels
Authors:Bruce Budowle
Affiliation:Forensic Science Research Group, Laboratory Division, FBI Academy, Quantico, VA 22135 U.S.A.
Abstract:Isoelectric focusing of extracts from diluted or aged bloodstains may be more successfully accomplished with larger sample volumes applied to the gel. A technique is described using teflon tubing to apply larger sample volumes (up to 100 μl) to isoelectric focusing (IEF) gels. This method is reproducible and easy to perform.
Keywords:Isoelectric focusing  Sample application  Phosphoglucomutase
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