| 鹅细小病毒VP1基因的克隆及序列分析 |
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| 引用本文： | 马波,王君伟,李洪涛,刘宝全. 鹅细小病毒VP1基因的克隆及序列分析[J]. 中国兽医科学, 2003, 33(12): 7-10 |
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| 作者姓名： | 马波 王君伟 李洪涛 刘宝全 |
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| 作者单位： | 东北农业大学,动物医学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 黑龙江“十五”攻关项目 (GB0 1B5 0 3 0 2 ) |
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| 摘 要： | 参照GenBank中收录的鹅细小病毒 (GPV)B株基因序列设计并合成了扩增GPVH1株VP1的 1对引物 ,利用PCR技术扩增出长约 2 .2kb的目的片段 ,将其克隆到 pMD 18 T载体上 ,进行了序列测定及分析。测序结果表明 ,GPVH1株VP1基因由 2 199个核苷酸组成 ,编码 732个氨基酸。经与B株、YG株进行同源性比较 ,核苷酸的同源性分别为 98.5 0 %和 93.18% ;推导的氨基酸同源性分别为 98.0 9%和 95 .77%。
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| 关 键 词： | 鹅细小病毒 VP1基因 克隆 序列分析 |
| 文章编号： | 1000-6419(2003)12-0007-04 |
| 修稿时间： | 2003-08-11 |
Cloning and sequence analysis of VP1 gene of goose parvovirus |
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| MA Bo,WANG Jun-wei,LI Hong-tao,LIU Bao-quan. Cloning and sequence analysis of VP1 gene of goose parvovirus[J]. Chinese Veterinary Science, 2003, 33(12): 7-10 |
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| Authors: | MA Bo WANG Jun-wei LI Hong-tao LIU Bao-quan |
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| Abstract: | A pair of primers was designed to amplify VP1 gene of GPV by PCR according to the published sequence of GPV B strain in GenBank. The product of PCR whose length is 2.2 kb was cloned to the pMD 18-T vector,then made sequencing and analyzing . The result of sequence showed that VP1 gene (included) 2 199 bp, which encoded 732 amino acids. The gene shared 98.50% and (93.18%) homology in (bases), and shared 98.09% and 95.77% respectively in amino acid with GPV strains B and YG. |
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| Keywords: | GPV VP1 gene cloning sequence analysis |
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